We narrowed to 286 results for: tac
-
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas ...
-
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m.... valid technical reasons to choose a non-Python stack, but if you're working with scientists it's unlikely... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m.... gender analysis for innovation and discovery. Tackling sex and gender in research Governments, universities... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....understand complex biological systems. He can be contacted at [email protected]. References 1. Cheng, Allen A... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....cell. Their Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....a gene while leaving other features of the gene intact. Here, we show an example gene targeted for tagging... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....expression manipulation. They are injected using a stereotactic frame into a defined brain region that is suspected... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m.... antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant in... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....indicators, these DNA constructs can be delivered to intact tissues and organs noninvasively and target a defined... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....higher risk cargo for a researcher to come into contact with than GFP. Similarly, if the viral vector carries... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....If the QC team identifies a QC issue, they will contact the depositing lab to review the discrepancies.... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....effects since promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions between... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....and having fun with technology, so feel free to contact your local space and get involved! Derek Jacoby... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....available near the locus you’d like to modify. To tackle the PAM problem, researchers have engineered alternative... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....target site, Cas9’s DNA binding capability remains intact while its nucleolytic activity is eliminated (Fig... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....or questions from your poster viewers and trade contact information if your conversation is particularly... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....portion can be bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....all disposable items and surfaces that come into contact with the rAAV and apply common cell culture practices...