...updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins...

...SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh...

...with inactive Cas enzymes can alter gene expression via acetylation or demethylation. Casilio DNA Methylation...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct... lower average Cas9-independent off-target DNA editing, ~5–50-fold lower levels of Cas9-dependent off-...Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus pasteurianus  or ...Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and...accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the...s lab identified and validated CBEs with reduced Cas9-independent deamination. They generated these editors...

...Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the Journal of Visualized Experiments (JOVE...and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines...motivations behind this research.   Using CRISPR/Cas9 for targeted genomic deletions We were inspired ...with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break...disrupted cells can be easily identified. Even in the case of clones with monoallelic deletion, the nondeleted...regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. J.Biol.Chem....

...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9...-wide profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187...Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional...techniques, please see “Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity” by Tycko et al. 2016...improving the genome-wide specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks ...high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing...of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9...

...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest...

...on Addgene/CLOVER viral vectors.  Caspedia: A data repository for Cas enzymes.  Open repository of CRISPR...

....2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation screening...

...     References and Resources References Ascoli CA, Aggeler B (2018) Overlooked benefits of using polyclonal...their ability to bind the target antigen. In this case, the serum is run over a column containing the target...

... gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve its multiplexing...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may... (15% and 9% of target sites.) How many loci can Cas9 modify? To test the application of this system to...