We narrowed to 614 results for: CaS;

...I’ve seen evidence of the infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of...

...Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment Simply expressing anti-CRISPR... group of proteins that are able to block CRISPR-Cas systems. They have been used to block genome editing....2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9 system for control of endogenous...applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility...specificity as full-length Cas9 (flCas9). When triggered by blue light (470nm), paCas9-1 induced indel mutations...proteins from N. crassa Split Cas9, Split Cas9 nickase, Split dCas9 Genome editing by NHEJ and HDR, repression...Photoswitchable Cas9 Richter et al., 2016 tsRC9 RsLOV from R. sphaeroides Cas9, dCas9 Gene editing ...

...updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins...

...2013). Multiplex genome engineering using CRISPR/Cas systems. Science (New York, N.Y.), 339(6121), 819...evolution of an adenine base editor with improved Cas domain compatibility and activity. Nature Biotechnology... engineering Cas9 variants with a preference for specific PAMs. Though near-PAMless Cas9s can broadly ...editing was invented in David Liu’s lab by combining Cas9 DNA-targeting technology with a fused cytidine deaminase...effects in a variety of RNA transcripts independent of Cas9 targeting (Rees et al., 2019). The Liu lab characterized... and eight bases, close to the PAM recognized by Cas9. If no PAM is available nearby, a base editor may...Kleinstiver’s lab engineered new variants of S. pyogenes Cas9. While the wild-type enzyme requires a PAM sequence...

...SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh...

...with inactive Cas enzymes can alter gene expression via acetylation or demethylation. Casilio DNA Methylation...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct... lower average Cas9-independent off-target DNA editing, ~5–50-fold lower levels of Cas9-dependent off-...Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus pasteurianus  or ...Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and...accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the...s lab identified and validated CBEs with reduced Cas9-independent deamination. They generated these editors...

...Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the Journal of Visualized Experiments (JOVE...and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines...motivations behind this research.   Using CRISPR/Cas9 for targeted genomic deletions We were inspired ...with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break...disrupted cells can be easily identified. Even in the case of clones with monoallelic deletion, the nondeleted...regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. J.Biol.Chem....

...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9...-wide profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187...Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional...techniques, please see “Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity” by Tycko et al. 2016...improving the genome-wide specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks ...high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing...of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9...

...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest...

...on Addgene/CLOVER viral vectors.  Caspedia: A data repository for Cas enzymes.  Open repository of CRISPR...