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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 928 results for: Gene

Showing: 111 - 120 of 928 results

  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...disease in which disruption of the smn1 gene results in the degeneration of spinal cord motor neurons. This...Schiavi will use Addgene plasmids to characterize smn1 interactions with other genes. He will use fluorescent...to identify candidate genes as drivers of peritoneal mesothelioma tumorigenesis. They plan to use CRISPR...assess their tumorigenesis. These experiments will serve to validate these candidate genes and allow Nabavi...19,000 live births (1).  While disruption of the smn1 gene is known to cause spinal muscular atrophy, the molecular...fluorescent proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans... Di Schiavi will be able to better choose which genes and pathways to target in future small molecule ...

  2. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...and can deliver large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas...evolution using bacteriophages. Researchers moved a gene required for phage propagation to a plasmid that... CAST integration of the promoter could turn the gene on. Once phage success was linked to CAST efficiency...new horizons of evoCAST In the emerging world of gene therapy, highly tailored treatments to specific ...In contrast, a system that can deliver a healthy gene as a single intervention for a variety of loss-of-function...evoCAST is an important step toward that kind of gene treatment. The researchers also compared evoCAST... (Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines prime editing with a site-specific...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular genome engineering tool offers... be incorporated into the bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases...adaptive immune system that utilized various cas genes not only to store a record of invading phages but...conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized. To date, 83% of...Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune system ...nucleases from re-identifying it (3). Surprisingly, few genes have been identified that neutralize CRISPR/Cas....Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas...

  4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...plasmid in the host bacterium that expresses gene III. Gene III is under the control of a T7 RNA polymerase... the expression of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria...activity. A plasmid-based genetic circuit in the bacteria links the expression of gene III to base editor activity... 1: David Liu gives a talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and...Cas domains beyond SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs...bacteria links the expression of gene III to base editor activity and editing speed and consists of the... active variant of the base editor, a functional gene III product is produced and can infect bacterial...

  5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...variants in a gene of poorly classified function and a well established cellular signaling gene, thus providing...above to demonstrate how gene expression profiling, systematic tumorigenesis assays, drug epistasis assays... be used as generalizable strategies to map the function of rare cancer variants and genes: High-throughput...bottleneck is that most recurrently mutated cancer genes are rare and most of the individual variants found...well-annotated cellular pathways). It wasn’t clear whether gene-agnostic bioassays could accurately test for molecular...numbers of cancer variants across a large number of genes as systematically as possible. In developing these...assays were designed to complement the one assay per gene approach that has been the historical standard. ...

  6. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...them together. Gene expression The reason for most viral experiments is to deliver a genetic cargo, typically...primary plasmid with the delivered cargo (often a gene) is called the transfer plasmid while the other ...component as well. On both sides of this promoter-gene combo are two long terminal repeats (LTRs). These...infiltrate the host cell and deposit viral cargo. Gene expression While AAV often does not integrate into...(more on this below), it’s still used to deliver gene expression constructs. Sandwiched between two inverted... AAV also requires some back up from adenovirus genes – E4, E2a, and VA – which are supplied on a helper...viral replication. More information on these viral genes is available in the adenovirus section below!   ...

  7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...the ccdB gene is replaced by the gene of interest. As a result, cells that retain the ccdB gene die off...tool. The plasmid pKSCTMF contains the mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR...antisense RNA regulates hok gene expression indirectly through the overlapping mok gene." Journal of molecular...associated genes. These systems better ensure that plasmids are passed down through generations (Figure ...application of CcdB is GatewayTM cloning, where the ccdB gene serves as a counterselectable marker in the destination...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...toxin-antitoxin system to construct a stable synthetic gene circuit that can be used over prolonged periods....

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest...Express a gene of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest...your choice of fluorescent fusion protein with your gene of interest: Plasmid Color Expression Description...Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric...Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric...monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused to the C-terminus of monomeric...C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...activators and repressors to target genes. Repressing target genes using dCas9-based repressors Early ...of the gene of interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating...activating target genes in a variety of species and cell types can be found on Addgene’s website. ...Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene.  Validated gRNAs are ... activate, repress, and even image your favorite gene. With so many Cas9 reagents available, it can be...introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic target for Cas9 and the...

  10. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... this gene. Should you cut it at the beginning of the coding sequence to delete the entire gene? Perhaps... in vitro, and using it in mouse embryos to generate gene modified mouse lines. Hopefully this post will...they have been generated (more on that later). Find Validated gRNAs to Target Mouse Genes To begin the ...chances of gene modification in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9...decide to do a gene modification experiment in mice to the day you have your F0 generation (barring life...Haoyi, et al. "One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated ...blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use...
Showing: 111 - 120 of 928 results