...scanned to verify that it is the correct sample. Tracking samples - it's all about location, location, location...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming... traced. If you’re just starting a lab, or are looking for a way to organize samples within your lab, ...in house if necessary.   Tips for barcoding and tracking samples 1. Start with the tube - Barcoded tubes... information in a small space and is ideal for tracking large collections. Many companies, such as Thermo...Although we’ve developed our own computer system for tracking samples, it can be done easily with a barcode ...multiple people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable...

...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent...we are highly collaborative, we love teamwork. Working collaboratively allows our team to best serve Addgene... example of agility at work is our process for working on a new feature. We start with a story that describes...best approach it in our code.  Sometimes while thinking through these steps, we discover we need additional...otherwise, this isn’t how we work. We all take turns working on different parts of the code. I think the best...we might have an EMTA theme and we will all be working in the EMTA area of the code. By having everyone...Modern Greek to be easily accessible today. Roughly speaking, we translated our software from Python 2.7 to...

... paper into an electronic environment and hence making it searchable and shareable, but some ELNs provide...community were to: (1) prevent vendor lock in by making it easy to get data out of the ELN; and (2) facilitate...repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility. Connectivity...references the record of research is incomplete, making it difficult or impossible to reproduce. As the...the graphic depicts, RSpace supports linking to all of the most commonly used online file storage tools...Slack conversations and import them into RSpace. The kind of informal conversations about research that can... are crucial to understanding research and the thinking and workflows that went into it. The ability to...

...growing quickly. Taking what I loved from each of these platforms (such as social networking, visual appeal... This post was contributed by Ryan Watkins, Professor at George Washington University and developer of...average video abstract view counts.” For researchers looking to garner more attention for their work, a video...discuss in-progress research, proposed research looking for funding, or are by researchers for whom English...English is a second or third language (making publishing their research all the more challenging even when...and let the world know about your science! Ryan Watkins is a professor at George Washington University ...the Addgene Blog 6 Tools & Tips: Online Social Networking for Scientists Read about the Evolution of Scientific...

...Harvard, Johns Hopkins, (most of) MIT and the University of Colorado are doing, and are checking with Yale ... salaried workers receive overtime payment for working more than 40 hours per week will increase from ...scientists within academia, most particularly postdocs working in the U.S., whose current salaries are below the...scientists are paid above this threshold or for tracking the hours that these scientists work and paying...Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed...across academia). Increased postdoc wages, overtime tracking? What are institutions doing? Institutions are...what their institutions, or institutions they're thinking about applying to, are doing - for example, your...

... most, get pleasure from taking up such complex challenges, quickly breaking them up into smaller achievable...clients. In this realm, there are a few different kinds of challenges that consultants get called upon to...healthcare consultant, I have worked on most of these kinds of projects. Almost all of my projects, have involved... The fast paced nature of projects often means working long hours both at home and at the office. Consulting...firms are increasingly aware of the toll that long working hours and the unpredictability of schedules can... career in healthcare consulting, I found that talking to alumni, friends, and friends of friends who ... an engaging way to learn about the trends and workings of the healthcare industry. Finally, on-campus...

...similar to that in samples of interest. If you are working with cells that are amenable to genetic knockout...knockdown approach instead. In this approach, you’re looking for an antibody that detects the target in the ...can therefore be helpful to try all three when looking to label an intracellular target.   Figure...background staining. This can include using: different blocking reagents; titration of antibody concentration ...flow cytometry through this approach. If you’re looking for a flow antibody, the YCharOS (Antibody Characterization...there is a cell treatment (e.g. PMA or a specific cytokine) known to either induce or suppress the expression... which may not be available to every scientist looking to do a flow cytometry experiment. But validation...

...know exactly what you are looking for and how many of that thing you are looking for, you are ready to begin..., it’s helpful to know how many copies you are looking for. Secondly, categorize your edit – is it any...that you won’t need to screen hundreds of clones looking for your edit of interest. For all of the screening...population. This way you can ensure you aren’t looking for a needle in a haystack before going on to generate...do. A popular screening method for knockouts is Tracking of Indels by Decomposition (TIDE) (Brinkman et... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon... software very similar to TIDE known as TIDER (Tracking of insertions, deletions, and recombination events...

...to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits...our baffled flight attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited...as an undergraduate, I never fooled myself into thinking I was an engineer. The talks we heard Monday night...discussed in the book). These synthetic biologists are taking the "genetic code" to a new level, changing it ...years. I kept busy for the whole poster session, talking to people from every conceivable background at ... Eric J. Perkins...

...Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable...Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory Coast where...very environmentally conscious friends who had me thinking about energy conservation even before it was a...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other... portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until I settled... hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered by the...