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We narrowed to 327 results for: aga

Showing: 221 - 240 of 327 results

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Celebrating 15 Years of Scientific Sharing

Type

Blog Post

Published

Jan. 14, 2019, 2:08 p.m.

...here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects...
New Tool for Lineage Tracing: The ClonTracer Library

Type

Blog Post

Published

Sept. 22, 2015, 2:30 p.m.

...the parent cell population using the library, propagated it, separated it into roughly identical replicate...
Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

Published

Sept. 24, 2019, 12:55 p.m.

...check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

Published

Sept. 4, 2018, 12:53 p.m.

...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...
Working with Nuclear Receptors

Type

Blog Post

Published

July 15, 2014, 4:03 p.m.

...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

Published

Aug. 11, 2016, 2:23 p.m.

...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

Published

July 14, 2016, 2:30 p.m.

... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

Published

Dec. 1, 2015, 3:30 p.m.

...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

Published

Nov. 19, 2015, 3:30 p.m.

...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

Published

June 14, 2018, 11:44 a.m.

...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

Published

May 22, 2018, 1:38 p.m.

...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...
Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Type

Blog Post

Published

May 1, 2018, 1:57 p.m.

...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

Published

May 7, 2020, 1:15 p.m.

...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...
Viral Vectors 101: Chemogenetics

Type

Blog Post

Published

July 11, 2023, 1:15 p.m.

...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...
Plasmids 101: The Wide World of Natural Plasmids

Type

Blog Post

Published

Oct. 8, 2024, 1:15 p.m.

...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

Published

Aug. 1, 2024, 1:15 p.m.

...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

Published

July 18, 2019, 12:55 p.m.

...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...
Hot Plasmids February 2024

Type

Blog Post

Published

Feb. 20, 2024, 2:45 p.m.

... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...
The Materials Science of Optogenetics Experiments

Type

Blog Post

Published

Sept. 17, 2015, 2:30 p.m.

...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

Published

Sept. 6, 2016, 2:30 p.m.

... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...

Showing: 221 - 240 of 327 results

Results per page:

Celebrating 15 Years of Scientific Sharing

New Tool for Lineage Tracing: The ClonTracer Library

Troubleshooting Your Plasmid Cloning Experiment

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Working with Nuclear Receptors

5 Tips for Troubleshooting Viral Transductions

Cpf1 Update: Comparison to Cas9 and NgAgo

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Illuminating Epigenetics with A FRET Based Biosensor

Adenoviral Vector Production and Troubleshooting

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Split-BioID: An Improved Method for Studying Protein-Protein Interactions

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Viral Vectors 101: Chemogenetics

Plasmids 101: The Wide World of Natural Plasmids

Antibodies 101: Stripping and Reprobing Western Blots

Open Resources and Plasmid Tools For Studying C. elegans

Hot Plasmids February 2024

The Materials Science of Optogenetics Experiments

Addgene's Cancer Collection Pages Connect the Research Community

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