We narrowed to 623 results for: SCR

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...

...that lab notebook from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

...Pol, the last component, contains the reverse transcriptase and integrase components to convert the cargo...the primary viral polymerase. E4/E1A activate transcription of other relevant viral genes (including the...

...single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies of their... such as a heterologous envelope or a post-transcriptional regulatory element can be used for either type...

...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli...

...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...Genome-Wide Control for Large-Scale Functional Screens        ...

... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

.... Such a system has been previously described for transcriptional activation/repression and genome editing...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

... fragments, these problems can be overcome by screening a higher number of potential clones. Golden Gate...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

...Cre-dependent gene expression – placing a series of transcriptional and translational termination features with... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

...from the associated paper:   Color Base FP Description Article (Link to Plasmids) PI Blue EBFP2...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes. ...

...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ...

...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

...forward as quickly as possible and submitted our manuscript to the journal Cell just six weeks later. The...Asian-, African- and American-lineages). Strain discrimination is especially important in the current outbreak...

...there are any forms that need to be signed, or manuscripts that need to be reviewed, bring them to the meeting...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...