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Showing: 441 - 480 of 507 results

  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

  13. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with...

  30. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...much information as possible from depositing laboratories and associated publications, but our quality...

  31. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

  32. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...

  33. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

  36. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can...

  37. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...

  38. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  40. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...
Showing: 441 - 480 of 507 results