Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 879 results for: IND

Showing: 441 - 480 of 879 results

  1. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Your Plasmids with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources...more hunting through a whole folder of papers to find the right experiment! The ReFigure extension also... to share their specialized knowledge of which findings are reproducible. For an example, see our ReFigures...and answering this 2 minute survey! You can also find ReFigure on Twitter (@re_figure), Facebook, and ...

  2. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...scientist’s career. We use writing to communicate our findings and share them with the world. If one doesn’t ...will be difficult or (more likely) impossible to find; therefore all the time spent collecting it will... course of the class, the moderator discussed individual sections of a paper, the abstract, figures, introduction...class, we met as a group for sessions covering individual portions of the manuscript. Stephen Blanke, Beronda...generating a quilt with your lab mates, you add the individual figures and results to make the final product...

  3. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...and you are smart, you can be a good manager. The kind of smarts and the type of skills that it takes to...what makes a good manager? First, it takes an open mind to be willing to learn and grow in skills. Managing... team. A strong sense of engagement in the work Behind-the-scenes support from their bosses and employers...miserable.   A good manager keeps these factors in mind at all times when making decisions, assignments ...their best work) Are continuous learners and have individual priorities for advancement in science Have their...

  4. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...learn how to be a scientist. Your PI should be the kind of mentor that will teach you how to: interpret ...Cepko to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have to ...interviewing you, you are also interviewing the lab to find a good “fit” and to ensure the PI will be a good...university websites, ResearchGate and LinkedIn to find past lab members. Most scientists love to talk about...mentors out there. With some good pre-work you can find a lab that will deserve your hard work and an advisor...

  5. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Otherwise we will all have to wait a lot longer for individual labs to come up with discoveries on their own...cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on Addgene’...based on that experience, soon directed himself to finding “immunological and virological solutions to crises... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...“The continuous creativity that Addgene takes to find ways to disrupt these inefficiencies is pretty remarkable...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...it easier for you to find and use the plasmids you need. Here's what you'll find in this article: An ...plasmids are always being added to the repository. To find all of the CRISPR plasmids available from Addgene...messenger-RBP sgRNA Library targets human mRNA binding proteins with 10 guide RNAs per gene. The lab modified...be applied for identification of essential MYC binding sites in a wide range of human cells.  A new pooled...

  7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus...Garcia-Beltran et al., 2021). Antibodies from individuals who received both doses of the Moderna or Pfizer... Figure 2: Sera used in the study came from individuals who received either one or two doses of the Pfizer...SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity. Cell 184:2372-2383.e9. https:...

  8. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory...condition known to the plant community as ‘plant blindness’ - the tendency for students to be more interested...and ordering platform soon to be released. For individuals interested in ordering from ABRC for the first...organizations such as the Center for Science and Industry (COSI) and the Franklin Park Conservatory. Do ...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...tags A number of epitope tags exist – all with individual optimizations and specific monoclonal antibodies...high success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...and the plasmids, technologies, and researchers behind them. As part of our thanks to you for being a ...Chari Cortez explains some of the basic science behind homology directed repair (HDR) and how it’s used...experiments and tired of having to PCR many colonies to find a successful clone? Addgenie Jessica Welch explains...Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous end joining (NHEJ)...Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition into ...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...of supplying a DNA construct, they could simply induce cells to take up protein directly? It’s not a new...delivery. D’Astolfo et al. coined the term iTOP (induced transduction by osmocytosis and propanebetaine)...showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly...Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR/Cas9 stem cell...674-690. doi:10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and...

  12. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...clinical; can we find ways to treat patients? Over a quarter of a million people have some kind of neural implant...instead of blue, which will allow researchers to independently control the activity of two populations of neurons... of progress. I would say we still would like to find ways to make these tools more selective for different...link in the Addgene Alerts box. References: Independent Optical Excitation of Distinct Neural Populations...

  13. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... but with the names reversed, meaning that the findings of the study were also reversed. The third group...participants read an abstract for a study with findings that challenged their beliefs, they were much ...catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990...gained from that research remains inaccessible, behind paywalls and in technical jargon that even scientists...References Drummond, C., & Fischhoff, B. (2017). Individuals with greater science literacy and education have...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools...expression of the array. After expression, the individual gRNAs are released by endogenous tRNA processing...which is followed by tRNA processing to release individual gRNAs for gene editing. They named this new array...-target editing and do not cause higher Cas9-independent off-target editing in comparison with single-...

  15. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the expert...been essential to push certain projects. The idea behind the platform was simple: any person working on ... collaborations are limited by the difficulty to find the right expert and the lack of credit for small...researchers who believe in making science less of an individual effort and more of a collective construction....research. It’s completely free, of course. You can find more information about us and what we do in our ...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...nucleic acid sequences that bind to the small molecule, DFHBI-1T to induce its fluorescence. They found...protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has ...genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR guide...

  17. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...you ask? Well, organizations used to negotiate individual MTAs every time they wanted to share materials...Addgene — basically, instead of having to sign individual MTAs for each request, the depositing organization...bit confusing, and it’s certainly frustrating to find out your plasmid hasn’t shipped because a form needs...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their...

  18. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist...proteins when missing, or edit inaccuracies as they find them (anyone can register with a valid email address...FPbase The most obvious use case for FPbase is finding information on a particular FP; this can be done...600nm?  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...

  19. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Libraries Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being... now know what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with...information I’ll be learning relate to me as an individual and Addgene as a company? What’s the key thing...

  20. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...shift can be prevented. Keep this information in mind if you’re planning on shipping something on dry ...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer... the samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting... at -80°C. We then thawed the tubes after the indicated incubation periods, and measured the pH. We found...Resources on Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

  21. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...opportunities available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after... are self organised, reliable, and can work independently. Practical outdoor skills are needed in a variety...currently in the position. Questions you’ll want to find answers to include: Will you be sitting at a desk... posts for more specifics on some careers and to find advice on crafting the perfect application. Additional...

  22. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...agile framework If you can’t find internships at science companies, try to find internships with translatable...relationship-building in order to rally many stakeholders behind a common vision. Use your network to your advantage... out the infographic for an outline of my day or find the text version below. 8:00 AM--Log on! My team...software and technology tools with the customer in mind. You can follow her on twitter @rgrubinstein. Additional...

  23. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control...presence of GFP to form a functional Cre enzyme that induces recombination of lox sites. This system takes advantage...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  24. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates, blocking...conjugates, or signaling molecules! Please keep in mind that this is just a brief introduction to the wide... if it ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive.... But PE can be very helpful if you're trying to find a relatively rare protein in an experiment with ...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  25. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...specialized franchise workshops throughout the year.  Find workshops in your area here: https://comscicon.com...out of our workshops. If you're having trouble finding science communication opportunities in your area...do, let us know!  To get in touch with ComSciCon, find us on Twitter, Facebook, Instragram, or send an ...works as a data scientist in the entertainment industry.       Additional resources on the Addgene Blog...

  26. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...regulation is complex and influences--directly or indirectly--many other areas of biological research. If ...field who have deposited with Addgene, but you’ll find many great tools by clicking on the links below ...below pull up lists of plasmids containing the indicated gene, and you can subscribe to these pages to ...to pages containing lists of plasmids from the indicated publications. You can request all of these plasmids...956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after...

  27. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... to find protocols that require reagents which aren’t broadly or easily available, so you may find yourself...learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may...type, and growth temperature) may need to be individually optimized for each of the three factors: the...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both ...

  28. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone...information or tools, making them useful tracers, but finding a virus that works well for retrograde tracing ...serotype in both mice and guinea pigs (work you can find in AAV Data Hub!) The cortical pathways to the IC...Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...Resources on Addgene.org Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus...

  29. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often...Screening libraries, large collections of biological independent variables, are becoming increasingly popular ...or maybe first!) viral experiment? Click Here to Find Viral Vector Resources at Addgene! References and...

  30. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization...are converted into emitted photons. These are indicated by the extinction coefficient (EC) and quantum... variation in the fluorescence intensities of individual mammalian cells within the dish. This is caused... brightness can be made (Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2...

  31. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Service Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics...Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of 2016. The... (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating...AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes...

  32. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...straightforward, but plasmid-based SDM has lagged behind. While commercial kits are available for making...GeneArt cloning - but at a much lower cost. Read on to find out how to replace your SDM method with REPLACR ...some of these methods, chances are you’d like to find a better one. Trehan et al. wondered if, instead... in which the phage recombination machinery is induced using L-arabinose. These cells can be made and ...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...

  33. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    .... ChIP-seq relies on antibodies to recognize and bind transcription factors directly, but that’s a slow-going... transcription factors and figure out where they bind to the genome. We’ve been doing that for years using...actually use this for tagging any protein in all kinds of different assays, not just transcription factors...two-fold: CRISPR/Cas9 offers high efficiency of inducing double strand breaks and more opportunity for ...Professors Mendenhall and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with...

  34. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...the cellular consequences of repressing or inducing individual transcripts. The activator (CRISPRa) sgRNA...plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow...really confident about working for most genes and individual targeting modules. Those two things, in my opinion...lentiviral CRISPR libraries and keep the following in mind: These libraries are pooled. Most of these screens...

  35. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...plasmid gets integrated into the host DNA or is independently replicated as an episome. The four main types... name suggests, these vectors can replicate independently of the yeast chromosome; however, they tend ...These vectors replicate as though they are small independent chromosomes and are thus typically found as a... size and A-T content of the DNA (apparently independent of a known specific sequence) dictate the replication... 5-FOA*. S. pombe  no no *FOA in fission yeast induces mutation in the ura5+ gene in addition to ura4+...

  36. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring domain), in electroporated in...where she engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput...archaerhodopsin‑derived genetically encoded voltage indicator which had improved performance in live mouse brains... Currently, she is using the improved voltage indicator coming from the screen to understand mouse cortical...Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex. Image from He Tian....

  37. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...irreproducibility. Luckily, there were other academics and like-minded organizations with similar ideas who were also ...bring together talented and motivated groups and individuals with similar ideas to actually do something about...impactful opportunities - There are many brilliant minds out there, but how do you get them to connect? Through... Through networking of course! Thanks to individuals in the group who were well connected, this diverse...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

  38. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization...reversible intensity changes (Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al,...simple mono-exponential decay (Shaner et al, 2008; Bindels et al, 2017) and cannot be described by a single...which far less power is used. For example you might find that you can get useful data from your chosen FP...

  39. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...and why should you learn more about it? Read on to find out how ccdB can make your cloning experiments a...gateway/introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator...developed specifically with ccdB-containing vectors in mind, any plasmid that contains the F plasmid (F’ strains...Bernard, P., et al. “The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase....Addgene.org Choose a Molecular Cloning Technique Find Plasmid Cloning Protocols Check Out All of Our Protocols...

  40. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...bench, we need to (this is a little simplified): Find the appropriate glycerol stock within our freezers...figure it out if they needed to, they’d have to individually keep track of any failures and do troubleshooting... are using barcodes (and you should be!) bear in mind that your barcodes are being applied to the containers...writing software tools to help scientists. You can find him on Twitter @krisshamloo and at krisshamloo.com...Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...
Showing: 441 - 480 of 879 results