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...the company and not just feel like an additional person each day. It felt like I could actually make connections...

...and decreased immune response to Cas9. Recently, Baisong Lu’s lab developed a lentiviral capsid-based bionanoparticle...

... and countless conversations both online and in-person, you helped us understand how Addgene can better...

...plasmid DNA. This an oldie but a goodie, and in my personal experience, is a quick way to generate many mutated...

...that comes from caring about your colleagues on a personal level, and employees often cite lunch as one of...

...Hoffman, Z. M., Gillis, J. E., Tjokrosurjo, Q., Markson, S. C., Huang, A. Y., Anekal, P. V., Montero Llopis...

...Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification...

...being featured in the National Geographic and Smithsonian Magazine, where the editor stated “Albert G. ...

...depositing scientists who weren’t able to join us in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke...

...occasional breather and acknowledge the team’s personal and professional growth along the way. Take time...

...only every science trainee had such a dedicated person guiding the start of their career. “I want to be...

...systems have been developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few...

... prioritizing research into this disease. A key reason this research has been able to progress so rapidly...

...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...

... Rachel Leeson...

... Rachel Leeson...

...  Rachel Leeson...

...than ever to get the support you need. If for some reason you can’t find the answer that you’re looking for...

...words of experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral...

...onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids...

... O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang...

...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...

...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...

...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

...protocol to remove endonucleases activity. For these reasons choosing the correct solution is fundamental, and...

...restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately chosen ...

...off-target effects.   New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

...it from anywhere. Its ease of use is one of the reasons why this was the first program I used to create...

...easy to spot and even easier to ship back. Be that person! If you use serological pipettes from Corning...

...This series was written for selfish reasons: I wanted to learn about careers in science communication...

...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...

...the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals...

...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

...tips for applying it in your own lab. Table 1: Comparison Between Standard Restriction Cloning and Fast...

...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

... Lianna Swanson...

...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...