We narrowed to 888 results for: URE

...antisense RNAs inhibit plasmid replication.   Figure 1: The replication problem. Iterons are repeated...the same RepA molecules, forming a “handcuff” structure that physically blocks replication machinery when...actually does, and neither plasmid is maintained (Figure 1).  The partitioning problem Plasmid incompatibility...leading to plasmid loss (Diaz et al., 2015).  Figure 2: The partitioning problem. For low copy plasmids...type of plasmid into a separate daughter cells (Figure 2) (Ebersbach et al., 2005). However, more recent...Partition-associated incompatibility caused by random assortment of pure plasmid clusters. Molecular Microbiology 56:1430...

...discipline to solve them on their own. According to a Nature News Article, since the 1980s researchers have ... of all of this appears to be that video is the future in terms of communicating ideas. In 2012, for example...and a few other disciplines leading the way, the future of complementing published research with video ... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5...pin/7439 Research Website: https://umaine.edu/aquaculture/ The goal of the WeShareScience project is to...Richard. "Interdisciplinary research by the numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring...

...experiments or even negatively impact cells (see Figure 1). When behaving properly, FPs enable researchers...inappropriate disulfide bonds in the secretory pathway (Figure 1) [4,6–8]. Additionally, FPs that encode N-glycosylation...from properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs containing two cysteine...experiments, please consider the following: How can one ensure that an FP fusion with a non-cytosolic protein ...cellular compartments including the secretory pathway (Figure 2), the inner membrane space of mitochondria and...proteins optimized for diverse cellular environments." Nature communications 6 (2015). Pubmed PMID: 26158227....

...bacteriophage lambda Can be temperature regulatable  Often paired with the temperature sensitive cI857 repressor...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the promoter...transcribed from the RNAP III promoters. This post features promoters for general RNAP II and RNAP III transcription...retroviral constructs and we will discuss these in a future post on viral vector parts. Promoter specificity...type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working in...

...below: How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s ..., we have also created video protocols.  These feature step-by-step instructions to navigate various lab...biotech nonprofit? In our Career Video series, we feature interviews with real Addgenies who have a variety...Addgene.  You’ll hear all about their career paths, future goals, and get some useful career advice.  Our ...

...applications.  Third, even in the most optimized tissue-culture based system, the efficiency of gene editing is...limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only...mutations of a gene into the endogenous allele in cultured cells. AAV genome editing combined with targeted...T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently...Hirata. "Human gene targeting by viral vectors." Nature genetics 18.4 (1998): 325. PubMed PMID: 9537413...editing via postnatal administration of AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017...

...desired variants of a protein. Applying a selective pressure winnows the pool down to plasmids expressing variants...High-throughput DNA sequencing is then used to measure the frequency of each variant during the selection...plasmid will be degraded by ExoIII. See the below figure for an example like this. 2) When your gene of ...to remove the BbvCI site(s). Overall, the key features of the one-pot saturation mutagenesis method are..."Plasmid-based one-pot saturation mutagenesis." Nature Methods 13.11 (2016): 928-30. PubMed PMID: 27723752... a Large Scale Using Deep Mutational Scanning.” Nature Protocols 9.9 (2014): 2267–2284. PubMed PMID: 25167058...

...Make sure to check out our validated gRNAs to avoid rolling the dice on a new gRNA in the future! References...target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In this...validation on your bulk population. This way you can ensure you aren’t looking for a needle in a haystack before...sgRNA-Cas9 combo. Pro tip: When designing your PCR, ensure there is at least ~200 base pairs of sequence flanking... inserted or deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you can...

...biomedical research and drug development. Integrin structure and conformational complexity There are 24 known...With the right research reagents, researchers can measure this conformational change to investigate the role...specific integrins in cellular processes.   Figure 1: Integrins can shape into three conformations...much lower affinity than their open states (C). Figure courtesy of IPI. IPI anti-integrin toolset IPI...biology talks on the IPI website and stay informed on future events by signing up for the IPI newsletter. The...characterization of subtype-specific β1 integrin mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038...

...Scientists. Back when I used to do mammalian cell culture, I would often respond to friends asking me to ...achieving your goals. However, you should have a good mixture of useful tasks that can be accomplished quickly...Monitoring task progress. For this, I always make sure I have explicit goals in mind for the endpoints ...extensively, my lack of desire to go sit in the tissue culture hood may have prevented me from splitting cells...splitting cells boring, hanging out in the tissue culture room also gave me time to listen to some of my ...any other time management suggestions, please be sure to note them in the comments! Additional Resources...

...to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are both much...defined three components required for translation (Figure 1): A tRNA that recognizes a specific ‘open’ codon...encoding the open reading frame (ORF) of interest (Figure 2). 1. The phosphoserine OTS The backbone of the...be optimized for parameters such as induction temperature and length of induction, which will vary between...Philip. "The origins of protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID:... permits programmable protein phosphorylation." Nature communications 6 (2015). PubMed PMID: 26350500....

...editing efficiency. Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery ...inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida...genome as a negative control. Read the paper in Nature Communications Find the E. coli genome-wide CRISPR...plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’s lab recently found ...

...perform transformations. A typical 200mL bacterial culture produces 20mL of competent cells, enough for 800...bacteria, the bacteria is typically grown in liquid culture without antibiotics to give the antibiotic resistance...plate your cells without adding plasmid) to make sure you don’t have contamination.  Also conduct a ...that transforms well in other competent cells) to ensure that your cells can be readily transformed. Compare...previous batches of homemade competent cells to ensure good efficiency.  Want even more tips for transforming...

...popular any one of them will become. One thing is for sure, as is obvious from our most requested plasmid, ...modified for viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This...included in this plasmid's name are some additional features. The first and most imporant is that, upstream...these variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References...Genome engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: ...

...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...strand breaks at target sites. Read the paper in Nature Communications Find the Cas12b plasmids  Plant...AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system...highly customizable as vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read...transferred via conjugation.   Read the paper in Nature Microbiology Find the Mobile-CRISPRi plasmids...

...Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...launched the cloning grade DNA service. This service features 210 plasmids: empty backbones, fluorescent protein...plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post. 9. Droplet...stably maintain them in the cell. This blog post features two mechanisms that cause plasmids to be incompatible...

...narrowly focused lectures, interactive panels, and a student poster session. All DayCon lecture content is ...variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs. Their website... engaged. The day kicked off with introductory lectures targeting three broad concepts: the genetic basis... what life is like as a student researcher. The future of DayCon DayCon and other similar events show ...

... toolkit SECURE adenine base editor Earlier this year, Keith Joung’s lab developed SECURE cytosine base...compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...RNA-recognition capabilities.  Read the paper in Nature Biotechnology Find the base editors   Single base...editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian cells. ...

...really made me think about my future. They made me rethink having a structured career because our mindsets...how to translate them into real world careers. I figured this internship would be a perfect way of exploring...different experience, It was helpful to have Addgenies reassure me that someone would always be there to help ...experience at Addgene, I am excited to see what my future looks like. ...

...climb upwards until they reach a point where the temperature and humidity favors fungal growth. The ants bites...out of the ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the...Vampirovibrio chlorellavorus will not grown in culture alone and relies on its algae host for reproduction...through its gills. It attaches there until they mature and changes sex from male to female. Then, it detaches...resources on the Addgene blog Read about company culture at Addgene See some of our previous Halloween costume...

...et al., 2024).   Figure 1: Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024...sequential knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038...cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038...in vivo screening of genes in the immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038...

... on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace has...personal development at the center of her lab (pictured above). DePace holds yearly planning meetings ...trainee to create a supportive, growth-oriented lab culture. The aim of these meetings is to give honest, constructive...PI-trainee communication builds a positive lab culture Although they may take some getting used to, yearly... lab culture...

...screens, but not to know even the life cycle of the creature they deal with.” So photography can be used as...2008. His work exposes and explores the hidden structures within flowers with the use of x-rays. His haunting...and have gathered much acclaim - including being featured in the National Geographic and Smithsonian Magazine...scientist with a new method to explore the inner structures of delicate plants and other organisms without...interest for its stunning images that is has been featured in Time magazine . Other smaller competitions ...

...It was immediately clear that Addgene wanted to ensure applicants understood what the expectations were...responsible for helping other scientists on the team ensure the deposits we received are what the depositing...for me at Addgene. There is a clear effort to make sure people feel connected.    As I became accustomed...aided by the efforts being put in by Addgene to ensure their employees are feeling comfortable.     Lucie... Tips for Writing a Good Cover Letter 7 Tips to Secure a STEAM Internship This Summer  ...

...nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR. A, Structural.... Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et al., 2023...enhanced response time allows for more precise measurement of norepinephrine uptake throughout the brain...indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038...ColabFold: Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038...

...They generated these editors using the BE4max architecture. The new editors reported in this study have... levels relative to BE4max.  Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi...CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells for...Enhanced CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout... methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you...

...called “self-service.” The idea here is to build features that allow users to help themselves, saving time... will continually update the Help Center to make sure that, whatever your support needs are, we’ve got...to the Help Center, you may have noticed a new feature on our website; towards the bottom right corner...www.addgene.org. We’re excited to make these new features available to the Addgene community, and we’d love...Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources at Addgene Visit...

...was delighted to be invited to the ceremony for surely what is one of many awards that will be bestowed...in markers on a whiteboard. The final image is pictured here…she started from a blank white slate and ...experimentation to the eventual translation to the clinic and cures. There is no doubt that companies are swarming to...computer to fight the known viruses. Bacteria have figured out how to download a bit of virus sequence to ...example, the technology is already available today to cure sickle cell anemia in stem cells but this can’t ...

...facilitating recovery of edited animals. Figure 1: In the background are 504 restriction digests... demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches...making screening for knock-ins much more efficient. Pure PCR repair templates are critical for efficient ...editing efficiency improved by using more dilute mixtures (Doksin et al., 2018). The Mello lab also reported...visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to note that recipients...and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89...

...discoveries, the world of academia often emphasizes structured methodologies and rigorous research plans. However...strategy could entail immersing yourself in diverse literature. By delving into new topics and expanding your...taking field trips, and spending time outdoors in nature or visiting museums can be excellent methods to... times (or use the notes app on your phone) to capture fleeting thoughts and connections that emerge during...recognizes marvel in all things.   Conclusion  In the structured world of lab research and PhD studies, where ...

...molecular biology toolbox organized, today’s post features a list of free online molecular biology tools ...provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might think of Benchling...Wayne Davis from the University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame...tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates a sequence...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...

...can be tricky, and it takes extra effort to make sure everyone in the meeting has an equal opportunity...emailing documents to one person at a time. Just be sure there is a point person responsible for incorporating...will come from the collaboration. Some things to figure out are timelines for experiments and manuscripts...from each other, access specialized equipment, or ensure that techniques are being performed the same way...feedback with everyone involved can speed up the future directions of the project. The state of research...

...invading phages but to also destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins... immune system seems like the winning ticket to ensure prokaryotic survival; however, viruses, too, are...strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome editing was..., Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy...Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L, Ran FA, Cox ...

...introduction, and a resolution.   Fig. 2: The structure of a story - not just for fiction! Image credit...more. This could be by outright stating the big-picture problem, by asking your audience a simple question...’t need to be anything fancy; it just needs to capture your audience’s attention enough to make them decide... new questions are next or how this will impact future research or policy.  It is worth mentioning that...patients or other affected people, man/animal vs nature, anthropomorphized animals, or even seemingly unrelated...

...we've been able to measure their relative ability to neutralize.”   Figure 2: Sera used in the...was not tested at the time of this study. Figure 1: Neutralization levels against spike pseudovirus...mutations was surprising,” says Balazs.   Figure 3: Neutralization outcomes of convalescent sera....V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021...

...🎶 “Bye bye L-arabinose drivePut your cultures in the shakerTurn the LEDs onAnd when you wantYou can ... a light-responsive domain that dimerizes upon exposure to blue light (Fig. 1). Another blue light-inducible...light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional BLADE variants, too...expression needed for most applications.   Figure 1: With blue light, the VVD domains of the BLADE...how spatially precise the activation is.   Figure 2: A bacteriograph of Michelangelo's "The Creation...

... lines (hybridomas) that secrete antibodies in culture and provide renewable reagents with reliable and...plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition, and...ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies using quantitative...Reproducibility: Standardize antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury... (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury...

...because it turns a dynamic process into a static measurement and it provides limited information about what...fluorescent autophagy reporters now allows for the measurement of autophagic flux, or the changes in autophagic...strength excited at 586 nm to 440 nm serves as a measure of autophagy induction: when Keima is in the cytosol...is high. When untargeted, Keima can be used to measure bulk autophagy, but the addition of a mitochondria-targeting...18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy in mammalian...

...2010). Rather than being released into the cell culture supernatant, the viral particles associate with.... Coroadinha, A. S., et al. "Effect of osmotic pressure on the production of retroviral vectors: Enhancement...overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering...Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but ...serotype-dependent release of functional vector into the culture medium during adeno-associated virus manufacturing...

...CPP-tagged protein and an untagged control could enter cultured cells and activate expression of a luciferase ...After iTOP treatment, both primary (hESCs) and cultured cells showed up to 70% resistance to diphtheria...enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances...elsewhere in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or...Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic ...