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We narrowed to 790 results for: Cre

Showing: 551 - 600 of 790 results

  1. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking...

  3. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...genetic diseases As CRISPR technology becomes increasingly more cost effective and precise, researchers...influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are ...

  6. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...in China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...the media and academic conference response to He created a positive feedback loop that rewarded unethical...

  8. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...two iodine residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The... KillerRed requires mitochondrial targeting to increase cell ablation efficiency, but dL5** phototoxocity...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin...

  10. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ...

  11. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

  12. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...

  13. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...

  16. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...

  17. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin...

  18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio...

  22. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...

  23. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

  24. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

  26. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

  27. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

  28. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...

  29. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

  30. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

  31. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

  32. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...

  33. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

  34. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  35. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

  36. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... off-target effects Prolonged Cas activity can increase the chance of off-target editing particularly ...Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem...

  38. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...participants can identify you. You may also wish to make creative use of a footer in your poster. This is a section...scientific poster, you shouldn’t hesitate to be creative. After all, your poster is a representation of...

  39. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  40. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  41. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  42. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  43. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...resulted in a cell migration defect. They have created a circRNA website so scientists can view public...developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system....

  44. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  45. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...discussion will get side-tracked, and you might create even more conflicts. 4) Define in advance how you...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...

  46. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... their restriction sites allowing you to easily create customized overhangs to assemble up to 6 different...number of fragments you’re hoping to assemble increases. Multiple inserts: There are some cloning methods...

  47. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

  49. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Pre-made viruses have become increasingly accessible and are useful for saving time and avoiding potentially... collected over the course of a couple days to increase yield. The collection process is a cycle of collecting...

  50. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...
Showing: 551 - 600 of 790 results