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TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...
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CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m.... Plasmid Serotype Name 50363 AAV5 AAV phSyn1(S)-DreO-bGHpA 121675 AAV9 pAAV-EF1a-fDIO-Cre (FLP-dependent... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....DNA repair. A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m.... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m.... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an...