We narrowed to 463 results for: BLI
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TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...
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Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...our pipeline (see Figure 2, sample 2). When we blindly analyzed AAV-68544, we were easily able to identify... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....subfields. Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests ...14%). Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...Collaborating with others outside of your organization Public Speaking Time management Managing a team Conflict... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....suppliers, and public health and medical organizations are working to help educate the public about proper... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....situation. So, young researchers need to publish good publications in a short period of time and may need... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...Janelia Research Campus on the GENIE website. Bibliography Ersing, I., Rego, M., Wang, C., Zhang, Y., ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m....Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....world of microbiology, some before they had even established their own labs. Fried chicken with a side of...worth addressing. Clear expectations have been established for future participants regarding the activities... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m.... from depositing laboratories and associated publications, but our quality control (QC) standards have...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...might help to get you started. Once you have established a well working protocol in your lab, it can become...