We narrowed to 584 results for: CHI;

...pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and implanting...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute... three germ layers, and the ability of theses chimaeras to produce all-iPSC derived offspring demonstrates...

...strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq), genome-wide identification and quantification...to test multiple expression systems before they achieve the appropriate expression level for their gene...cloning process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily,...genome-engineering endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically...

...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...

...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....

...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...

...organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, ...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

... your own depositor metrics!  About the Author Joachim Goedhart is an assistant professor at the Section...fluorescent probes. You can follow him on twitter: @joachimgoedhart.    ...

...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk...Fireplace from the Dyer Lab If you’re feeling as chilly this winter as the Addgenies are in Massachusetts...

...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have been possible...

...was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the entire blog and ... Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always...

...lab equipment to carefully-screened research and teaching departments at universities around the world. ...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the...

... DSHB was created by the National Institute for Child Health and Human Development (NICHD) in 1986, to...toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein targets in...

...establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions...

... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...

...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...

... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...