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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 221 - 240 of 659 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ..., 1998), this 15 amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...choosing a dye or ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new...

  2. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...this time, it is! Factorial ANOVAs include two-way ANOVAs but can include many more than two independent ... Of course, a one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...

  5. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...on immune system Conventional cancer therapies include radiation therapy (owing to contributions from ...blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73 on specific... factors researchers are beginning to focus on include: Cell heterogeneity Tumors are composed of a wide...

  6. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Science is about fighting scientific partisanship, including more scientists in our leadership, and raising... Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health policy....Committee about climate change. All the scientists – including global warming skeptics – insisted that more data...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...

  8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for arbitrary... and return later or share them. Community exclusivity: Different communities develop software for their...software is organism agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Scorpions Low Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  ...Low Reporter and quencher on hairpin structure included in primer Yes Yes Agarose gel Table...well-to-well normalization of signal. They are often included in commercial sets and most machines have a protocol...

  10. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....

  13. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...

  14. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery yet...name! Additionally, anyone whose submission is included in the final four will receive some rockin’ newly...

  15. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...primarily been performed in activated T cells, precluding analyses of T cell differentiation beginning ...also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...

  19. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...

  20. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...
Showing: 221 - 240 of 659 results