...Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other than a white... cursory manner, their inclusion here adds much needed context to understand every other problem. For ...detract from the quality of the book? It doesn’t need to. The problems and challenges described in The...your life, the book may be the introduction they need to grasp the challenges you face in your career....

...Of course, if qPCR were perfect there would be no need for ddPCR. One issue with qPCR is that the results...a small number of qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning...droplets) can be sufficient to capture the information needed for your PCR experiment. The process to titrate...simultaneously. For AAV titration, you should only need to use one. However, two channels can be used to...

...established grant professionals is learning to succeed in grants through trial and error, through winning...likelihood. We provide expertise on the processes needed to successfully implement, manage and close-out...materials for publishers. When the community orchestra needed a grant writer, Deb volunteered. Through these ...private businesses. How do you get the expertise needed to enter the grants profession? The four steps ...

...delivery system, the specific target cell or tissue needs to be considered. Q2: Can I use lentiviral transfer...When expressing a viral construct transiently care needs to be taken to ensure that the proper cell line ... 5’ LTR of the transfer plasmid to eliminate the need for the HIV Tat protein and consequently are considered...where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids replication...

...location and type of edit you need to introduce highly specific? Perhaps you need to introduce a single nucleotide... have little flexibility as to where your sgRNA needs to be located and there may not be a NGG PAM site... and published in peer reviewed journals. If you need to generate a new sgRNA for your experiment, select...

...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...Because rabies dG can’t infect neurons on its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA...pseudotyping that could be broad or specific to suit your needs. Check out some of these review articles to find...https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ...

...updated pEcCas/pEcgRNA system, and confirmed that indeed the gRNA-pMB1 had a slightly higher leaky expression...MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids (pDonor and pQCascade... strain library, our pEcCas/pEcgRNA system would need to construct 13 plasmids with 14 rounds of transformation...of these systems a try, we’d love to hear your feedback!   Thank you to our guest bloggers! Qi Li obtained...

... modify the protocol to best fit their specific needs. Herein, we’ll give you a rundown of the different...directly conjugated to the reporter, there is no need for a secondary antibody incubation step, saving... the signal. Since the primary antibody does not need to be conjugated, and conjugated secondary antibodies...feel empowered to choose the best ELISA for your needs. Good luck and happy binding! More resources on...

... given set of resources, time, and experimental needs. Since its invention in the late 1990s, Gateway ...easily select for the expression clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. ...clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation of the...set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products directly...

...issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed raising the salary...for Dec 1 2016. In order to be exempted from the need to pay employees overtime, salaries must be above... of $23,660. Any postdocs on such a salary would need to have their salary raised 101% if hours are not...make gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a...

...antibody host species and isotypes. Consequently, users need to choose the protein type that is most compatible...those in the nucleus, more stringent buffers may be needed. Since many factors, including salt concentration...strength and pH, affect binding the lysis buffer may need to be adjusted depending on the sample. It is important...be translatable to the endogenous system and will need to be verified using alternative methods.  Finally...

...of codons, it only needs to produce a subset of charged tRNAs and therefore may need fewer resources for...Adaptation to changing conditions - Organisms often need to express genes at different levels under different...determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences 107.8 (2010...

...initially created to support the CRISPR analysis needs of Synthego’s scientists, and it’s now free for ...experiments and comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE...ICE will do the rest. There are no parameters that need optimizing and no complicated steps to learn. For...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...

...scientists as well as other citizens. Thus civilization proceeds in an informed fashion. For science to be useful...representative in the House of Representatives all you need to know is your area code. Senators: To find contact...contact information for your senators all you need to do is select your state. State Legislatures: To find...Trends in Federal R&D NJ.com: Rush Holt: Lawmakers need to use a scientific approach to formulating views...

...reduce non-specific binding of antibodies.  You may need to optimize the type of block used, the concentration...which will bind to your target molecule. You may need to optimize the concentration and incubation time...antibody and is conjugated to a fluorophore. You may need to optimize the concentration and incubation time...antibody seems and what sort of protocol you will need to use. (To learn more about antibody validation...

...organism; they are relatively simple to maintain and breed and they have large, easily modifiable embryos, ...frog research is The National Xenopus Resource, a breeding facility for X. laevis and X. tropicalis, the ...development, a large number of offspring (produces many seeds), self fertilizing, a small yet highly characterized...Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms. Dong wu xue yan...

...undesired mutations through the PCR process, you need a high-fidelity polymerase. There are many high ...high fidelty polymerases on the market; you need one with 5’->3’ polymerase activity (for amplification),...potentially truncate the template). Importantly, you also need a DNA polymerase that produces blunt-ended PCR products...DNA duplex stability from the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986...

...fluorometric-based detection. This increased sequencing speed ultimately leading to what are generally considered...became the workhorses of molecular biology." Proceedings of the National Academy of Sciences 102.17 (2005...restriction endonuclease of Hemophilus influenzae." Proceedings of the National Academy of Sciences 68.12 (1971...sequencing with chain-terminating inhibitors." Proceedings of the National Academy of Sciences 74.12 (1977...

...choice as shown in the figure below. To start, you need to have DNA fragments with regions of homology at...step without scars as depicted below. First, you need to design primers to amplify the two fragments while...is typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding ...fragments. CRISPR cutting was used to circumvent the need to PCR amplify the fragments of the chromosome, ...

...previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works...provided neuroscientists with the vast array of colors needed to mark individual neurons, the system also suffered...distinct cell populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes...provides spatial and temporal control without the need for germline modification, and enables Brainbow ...