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We narrowed to 824 results for: EED

Showing: 1 - 20 of 824 results

  1. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...Guidelines for giving feedback Use formal and informal mechanisms for feedback (more on this below). ...management style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to...well. Find out the Effect of the Feedback: How does this feedback compare with your own perception? ...What help did you need? What help did you solicit? When did you first see you needed help? What kept...the way to go when giving feedback. The employee is not helped if the feedback session is over and they...a 5 part series on Management for scientists. Feedback is help and it should result in authentic assistance...effective and successful. Giving timely and useful feedback is an absolutely required aspect of being a successful...

  2. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...that brings feedback back to the manager. Team members rarely rush to give honest feedback to the manager...these); ask for feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats...changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty quickly....communication especially important for a manager – getting feedback from the team. Successful management can almost...to learn about her management style.   Seeking feedback frequently One of the most important paths of ... variety of reasons. They may feel that giving feedback will be perceived as complaining. They may worry...successful manager that is genuinely receptive to feedback and so receives this valuable input from at least...

  3. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...busy scientist working hard on research, and you need the right experimental materials. Who has time for...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...complementary DNA strand. These 10 nucleotides are the “seed” region of the RNA guide, where exact matches to... gray.   Once it has found a PAM site, Cas9 needs to check for complementarity with its guide RNA....pair to be unwound. A mismatch within the 10-base seed sequence will destabilize the nascent heteroduplex...the next 8-10 bases after perfect pairing in the seed sequence.   Figure 6: Electron microscopy...cleaving it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork...

  6. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...mice can be included in breeding strategies identical to those used for breeding heterozygous mice. In ...all of the offspring in the F1 generation, your breeding scheme should start with parental mice that are...detect your targeting vector or gene of interest. 2. Breed your parental mice to generate F1 Once you’ve performed...when hemizygous, meaning that only one chromosome needs to contain the transgene in order to see an effect...locus (e.g. Cre) and heterozygous at the other. 3. Breed your F1 generation Now, your goal is to generate...as progeny from future generations. 4. Continue breeding your colony You’ll want to generate enough mice...harbor similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...

  11. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ... few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to researchers in...awards has successfully concluded, Addgene and Seeding Labs will be opening applications for a second ...researchers who have received equipment through Seeding Labs’ Instrumental Access program. We are excited... Resources Apply to Recieve Equipment through Seeding Labs' Instrumental Access Program Additional Resources... seeding labs...

  12. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ... can get involved with Seeding Labs Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional... that need to face these challenges locally. We look forward to more collaborations with Seeding Labs ... proud to announce that we are teaming up with Seeding Labs to better serve those in developing countries...Developing an international scientific community Seeding Labs, a nonprofit founded in 2007, strives to expand...Through its flagship program, Instrumental Access, Seeding Labs partners with donors to supply surplus lab...and talent is just a first step; going forward, Seeding Labs hopes to expand its activities by facilitating...more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important...

  16. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ... the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...More About the Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees... the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists .... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

  17. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...hard to appreciate without computational support. Indeed, rational sgRNA design is not possible without ...was subjectively ascribed relative to the basic needs of each individual; the  community as a whole has... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...collaborative effort from the community in order to succeed. With respect to de novo sgRNA design, I again ...you to select the tool(s) based on your project needs. There are 8 major categories found in the table...Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...Different communities develop software for their own needs. This often results in software design that is applicable...

  18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding Labs Plasmid Grant Awardees...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers...researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce ...modify the course of this condition are urgently needed. Therefore, understanding neuronal networks, their.... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

  19. Plan Your Experiment

    Type
    Collection
    ...population need to be identical (clonal) or is a mixed population okay? Does the edit need to be homozygous...interference Needs good primer design Western blot Knockouts Activation/interference Needs a good antibody...other organisms. As with most experiments, you first need to have a biological question in mind. Then you ...will help you narrow down which components you’ll need for a given experiment. In the table below, we highlight...dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription ...HEK293) cells. For more difficult cell types, you may need to look into other options. Browse Addgene's collection...cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, you directly...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9...45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles , Harrison , Wildonger...
Showing: 1 - 20 of 824 results