We narrowed to 513 results for: Emb
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TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....micrograms of starting material; Gibson assembly or Golden gate assembly use a more direct approach, allowing...
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Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....are coming along at a fast and furious pace. In December, as we've blogged about before, there was the ...via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) ... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR), developed... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog PostPublishedJuly 7, 2017, 1:32 p.m....how it's used. Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing ...stick to our core mission. Chapter 13: Outro - remember to step back and ask, "Why did I start doing this... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....to specific drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As ... described in the journal Science Signaling in December 2014, he and his colleagues at Duke University... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and S-CHIME... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....create Phage Directory in a whirlwind three days in November 2017. #Phage researchers! I am working with...Strathdee, 🗡️Superbug Slayer 🗡️ (@chngin_the_wrld) November 8, 2017 Facilitating the search of phages for... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m.... former Co-director of Science in the News, and member of the SITN podcast team. You can follow Vini on...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in...appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....benefits for both PI and trainee. DePace and her lab members have noticed that the meetings make the lab run...the PI has an opportunity to check in with lab members, determine how to work through obstacles, and take... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive...integral part in its success. The Addgene board members were also recognized for their essential advice... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....filled with family, food, and travel, and you can’t remember where you put your flow cytometry protocol. Or...Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....participate in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in...the beginning, I spent a lot of time shadowing members of my team to learn what I was to do for my time... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the impressively...F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently tagged versions... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org Plasmids... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....performed with a probe surrounded by a semipermeable membrane, which is inserted into the region of the brain...passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the perfused... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....insights they glean from a reading session. To remember what happened when drug X was added to cell line...with a specific gene knock out, often you have to remember the experiment, not the paper. To get around reading... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....came to visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run ..., the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family, including... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...