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  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

  3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... more than 80-85% confluent because cells like their space. If they reach confluence, they stop growing...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...types used for packaging viral particles (6), can influence vector performance. Open science initiatives, ...how small changes in vector administration can influence AAV expression properties.  By providing many ...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA in untreated capillary. Electrophoresis...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying this mantra to...and the effects of metabotropic and ionotropic influences on overall cell function. AAVs are the most appropriate...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until ...interest is expressed together with a marker (Fluorescent protein or selection marker) allowing for selection...

  16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...ligation product in a gel, but we can now use fluorophore- quencher pairs or qPCR-based methods to greatly..."Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging system; or...100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,000. Secondary...

  18. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com/pYBL3aLK4f...

  19. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices Paul...

  20. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...We have Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and...
Showing: 221 - 240 of 378 results