We narrowed to 388 results for: FLU

...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...

... relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...

...this by changing the voltage for the particular fluorophore prior to recording data. Increasing the voltage...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

...including FokI endonuclease domain as well as fluorescent proteins used to sort the cells after the plasmids...neurons, for instance – to see how those mutations influence the disease process. In the Cell Stem Cell paper...

...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

...in HeLa and MEF cells. For further reading on fluorescent microscopy techniques, the first author of this...El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4...

...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only...

...that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

...for monitoring homemade microfluidic chips using the fantastic optics and fluidics platform it contains....

... more than 80-85% confluent because cells like their space. If they reach confluence, they stop growing...

...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...

...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

...types used for packaging viral particles (6), can influence vector performance. Open science initiatives, ...how small changes in vector administration can influence AAV expression properties.  By providing many ...

...tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to observe changes...about zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

... pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g....

... manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank... process is expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants...

... a minimal promoter to drive expression of a fluorescent gene and packaged into viral vectors that can... To evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned...

...overlapping AAV since both of these variables influence whether cells will be co-transduced with multiple...Drawbacks: The choice of homology sequence can influence the level of transgene expression, and may need...

...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA in untreated capillary. Electrophoresis...

...ligation product in a gel, but we can now use fluorophore- quencher pairs or qPCR-based methods to greatly..."Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating...