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We narrowed to 391 results for: FLU

Showing: 221 - 240 of 391 results
  1. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...
  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...amplify each TF to determine how its presence influences cell fates. The atlas of directed differentiation...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...
  3. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...this by changing the voltage for the particular fluorophore prior to recording data. Increasing the voltage...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...
  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...including FokI endonuclease domain as well as fluorescent proteins used to sort the cells after the plasmids...neurons, for instance – to see how those mutations influence the disease process. In the Cell Stem Cell paper...
  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...
  6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...
  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... more than 80-85% confluent because cells like their space. If they reach confluence, they stop growing...
  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...
  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...
  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...types used for packaging viral particles (6), can influence vector performance. Open science initiatives, ...how small changes in vector administration can influence AAV expression properties.  By providing many ...
Showing: 221 - 240 of 391 results