We narrowed to 285 results for: Fus

1. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...occur), deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case...

2. Working with Nuclear Receptors

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   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...both of endocrine and dietary origin) that are diffused or actively transported from cells, traverse the...

3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501...

4. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

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   Jan. 5, 2021, 2:15 p.m.

   ...heterogeneous cultures.  HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)...

5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...spatial control of CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows...

6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...

8. A Look at Addgene's QC Process

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   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ... vector type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids...

9. CRISPR in the Clinic

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   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

10. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...complicated. These strains are more picky eaters and refuse to eat liver paste mixed with E. coli. Thus, to...

12. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow...

13. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ... In addition to generating fluorescent protein fusions, plasmids are available for tagging with epitope...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...

15. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

16. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable...

17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...

18. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...