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We narrowed to 878 results for: IND

Showing: 221 - 240 of 878 results

  1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... over the world, and the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift...proteins play an important role in RNA interference, binding RNA guides to cleave foreign RNA. Argonaute proteins...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9...observe differences in cleavage efficiency. If this finding is replicated in larger studies, it will represent... If you’re looking to disrupt gene function via indel formation, this property could be an advantage. ...fidelity would prevent other small nuclear DNAs from binding NgAgo, it might make it difficult to use NgAgo ...Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...These RNA duplexes bind to the RNA-induced silencing complex (RISC). After binding to RISC, the RNA duplex...strand usually being degraded. The target strand binds to the mRNA, which is then cleaved by RISC and degraded...Argonaute protein. This Argonaute protein is what binds the small RNA and either cleaves the target mRNA...sequence is highlighted in green, with red boxes indicating mature siRNA. Hairpin loop is in between the ...sequence is highlighted in green, with red boxes indicating mature miRNA. Hairpin loop is in between the ...stable knockdown cells lines, and you can usually find validated shRNA sequences for your intended target...RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43...

  3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...interest. Any proteins that bind the RNA motif will be biotinylated.  Find more details about RapID! EXCELL...biotin. Biotin, is a natural coenzyme, that strongly binds the glycoprotein, avidin or a similar protein, streptavidin...biotin molecules to dissociate from the ligase and bind to exposed lysines on neighboring proteins. Thus...organoid or in vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development ...biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino acids) found in the E. coli version...fusion proteins to specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate...Lam et al. 2014). They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid ...

  4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...known as steric hindrance. For instance, GFP may occlude catalytic sites or obstruct binding sites or motifs...POI). To indicate the sequence, people often use terminology like “C-terminal fusion,” but I find this very...increases avidity because it doubles the number of binding sites per unit.  There are several ways to measure...applications, many were concerned with steric hindrance by the fluorescent protein. Thus, scientist began...Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking... PubMed Central PMCID: PMC6381139. Costantini, Lindsey M., et al. "Assessing the tendency of fluorescent...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause frameshifts...Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research...concept for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections... function. Without functioning dystrophin, an individual experiences progressive muscle wasting leading..., but at least partially functional protein. Individuals with these mutations are often asymptomatic, .... With regard to off-target effects, each group finds very low to no off-target activity at the ten highest-ranking...cause obvious germline modification, an important finding given the controversy associated with such editing...

  6. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...science on. Practice self-care and stay connected Find time for exercise. Many studios and gyms are now... to support your favorite local exercise spot or find something new. We usually have yoga class twice ...great activity for both exercise and clearing your mind. And because the outdoors is so huge, these are ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...Work on personal development Take the myIDP (individual development plan) from Science to identify skills...and set up activities that kids can do more independently. For example, they could do something like puzzling... kids. Plan out meals and snacks ahead of time.  Find learning activities for kids. For example, Skype...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...at Addgene.org Find Fluorescent Protein Biosensors Browse All Fluorescent Proteins Find AAV Tools References...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations...anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein CAG: Generic strong... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  8. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding protein so ...are able to quickly sequence an individual’s genome. But when they find genetic variants, what does that...Get the basics on GFP Find microbiology blog posts Resources on Addgene.org Find fluorescent protein ...” Unger began with the a bacterial periplasmic binding protein, one similar to the protein which gave ...bioreporter such that fluorescent proteins expression is induced prior to inoculation with IPTG. In the absence ...

  9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday...award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins (FLiPs) Fluorescent proteins can be applied...these photoswitchable enzymes Fluorescent Light-inducible Proteins, or FLiPs.  Xin later optimized these...spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She hopes that other researchers...create a wide variety of enzymes and proteins with inducible activity and recently presented her work at the...sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima specifically proposes...with bacteria engineered to produce HMOs, sensor binding results in an increase in fluorescence of cells...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...buffers to use and that indicates if a sequential digest is needed. The enzyme finder is also a very helpful...learning proper cell culture technique, I tried to find an app that could help me with calculations. This...to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest. Lastly...with research! It is always important to know what kind of reagents you work with in experiments. Hence,...app has a search function that allows the user to find an MSDS for a lab chemical of choice. Now, I can...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource to..., J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split Calculator by ...

  11. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...cannot offer. Furthermore, companies across most industries tend to hire their former interns to fill entry-level... in.     STEAMid is an app that helps you find internships and scholarships in different subject...to submission. When in doubt, consult the web to find the tools that can help you craft a solid personal...Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle to vouch for the ... superb and detailed portrayal of yourself. Be mindful that January and February represent peak season...for internship applications. Thus these noble individuals are inundated with requests. The easier you make...        5. Application submission  Set a calendar reminder for every deadline and shoot to submit your application...

  12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...selection strategy is not enough on its own to help you find your desired plasmid. In many cases, you will need...survive and you will need to sort through them to find the desired clone. Do your selection, then screen...𝛽-galactosidase, it turns into an insoluble blue pigment. Find plasmids for blue-white screening at Addgene! Some...proper positive and negative controls for your PCR.  Find the details and learn more about colony PCR in this...into your insert. Check out our Vector Database to find plasmid maps of the more commonly used backbones...you can use to confirm your clones. You can also find a list of popular sequencing primers in our Molecular...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...functions. Photoswitches are engineered molecules that induce conformational changes with light stimulation to...activation have been developed to selectively study individual types of GPCRs with greater spatial and temporal...of the receptor, similar to what the endogenous binding partner would do. This allows precise control over...molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor that change shape...concept, except the ligand is attached to a protein binding partner or nanobody that targets the native receptor... when exposed to light, the ligand engages the binding pocket and activates the receptor. Created with...absence of light the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, so be...different ways to ultimately achieve success, we find it is usually faster and easier to do the validation...it can be ubiquitous, cell-type selective, or inducible.  Ubiquitous promoters  These often-strong promoters...over time, resulting in decreased expression (Damdindorj et al., 2014; Nieuwenhuis et al., 2021). It is... Attribution 4.0 International License.   Inducible promoters  This type of promoter allows you to... turn off, gene expression by administering an inducing agent like doxycycline (Favre et al., 2002). They...experimental output. You’ll also want to keep in mind the serotype you’ve picked, since that can affect...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...controls.) 27.6% of embryos carried indels at the mutation site, indicating that repair proceeded through NHEJ...move forward from this work. Experiments & major findings Let’s start with the paper itself: Ma et al. were...electroporated them with an ssODN repair template in induced pluripotent stem cells made from the patient. With...allowing repair to take place before DNA replication. Indeed, they observed that 41/42 embryos were non-mosaic...using the provided ssODN template. In fact, this finding led the authors to title the paper “Correction ...pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing...pregnancy, the researchers were able to examine each individual blastomere to determine its genotype. However...

  16. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...on the Addgene Website Find CRISPR Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources...combined with the weak separation afforded by gRNA binding at non-target sites would theoretically decrease... Figure 1: Strand separation and target DNA binding to the Cas9 nuclease. Stable strand separation ...similarly thought that if they could weaken sequence independent interactions between Cas9 and DNA, then they ...SpCas9-HF1 each stall in an inactive form after binding to a target site and propose that this stalling...Science (2015): aad5227. PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu...effects." Nature (2016).  PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene Find the ClonTracer...exciting new tool that allows researchers to individually label millions of mammalian cells through lentiviral...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...these groups with the same drug regimen, they would find one of two outcomes (see schematic depiction below...ClonTracer library allows a researcher to label individual members of a starting population of cells with...multiplicity of infection (MOI) such that each individual cell receives only one barcode. The abundance...is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to ...

  18. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual switch regions...PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...-lox system is a technology that can be used to induce site-specific recombination events. The system ...key advantage of the system is that Cre acts independently of any other accessory proteins or co-factors...recognition sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric ...certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre...unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence...

  19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same three nucleotides...Fidelity, it turns out, might depend more on the individual sequence(s) than on how well that sequence fits...Pryor et al., 2020), and randomly selected non-palindromic pairs allows for high-fidelity assembly for up...potential for mismatches, so you can redesign individual pieces as needed to improve fidelity. NEBridge...tool for you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...define the number of fragments, and the “search windows” you’d like the tool to use to identify fusion ... return the highest-fidelity overhang set it can find for those parameters. For even more specificity,...

  20. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...historical ties to psychology and philosophy (the mind-body problem). Because of the availability of tools...between animals as olfactory stimuli that were left behind from a preceding animal (ex: urine) can be distracting...Animal Strain, age, sex, housing conditions (individually versus in groups), health status, diet, cage...differences in behavior observation); experimenter blinding to avoid bias Genetic manipulation in behavioral... neuronal targeting after stereotaxic injection. Find more details about choosing AAV or lentiviral vectors...about two weeks before the behavior test. This time window between injection and testing will vary depending...
Showing: 221 - 240 of 878 results