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We narrowed to 791 results for: Kin

Showing: 221 - 240 of 791 results

  1. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...much of the primary data one might require when making such a decision remains spread across literature...With thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized...building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published in the last...suggestions. If you’re not sure exactly what you’re looking for, the Table of Fluorescent Protein Properties...that set of proteins.  For computer-savvy users looking to incorporate FPbase data in their own projects...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...important part of being a “good scientific citizen” and making sure you do what you can to help your fellow researchers...consistent way so other researchers can use them, to making sure that methods and protocols are available and... can be published. To a scientist, all of this checking and formatting can feel like yet another hurdle...for being transparent in their communication or making their data and reagents readily available. What...confident that everyone’s enthusiasm for actually making it happen would increase dramatically. I’m not ...

  3. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...requested dozens of times in 2 weeks. This is a striking example of the contributions that scientists are...to shared materials, and then pay it forward by making the new materials they develop available to the...has been able to rapidly propagate this strain, making it available to scientists around the world. This...research by removing logistic hurdles to continue making materials available rapidly to scientists (Nature...Yuen K-Y (2020) Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV...

  4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...culture towards a more generous and rewarding way of making science.   How we started Crowdfight-COVID19...as a collaborative platform to help researchers working on COVID-19. Its origins trace back to March 2020...-19, an online platform to connect researchers working on COVID-19 with scientists of all areas who volunteered...idea behind the platform was simple: any person working on COVID-19 could file a request on our website...We are a community of researchers who believe in making science less of an individual effort and more of...

  5. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University...University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria...research related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology...be able to support diverse groups of scientists working in a wide range of research fields and geographical...

  6. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... account, you can begin the deposit process by clicking "Submit Online Deposit" (for online submission... menu: Figure 1: Begin the deposit process by clicking "Submit Online Deposit" or "Download Deposit Spreadsheet...  Figure 4: Add plasmids to the article by clicking “Add Plasmid to this Article” (left) and entering...button and jump around to the different pages by clicking on the appropriate boxes at the top of the page...the plasmid to copy from the drop-down menu and clicking the white "Copy" button. *Note: The "Copy" button...Submit Deposit" button to complete the data entry. Clicking the "Submit Deposit" button will allow you to ...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...something is working for you, then there is no reason to change it. But what if a kit isn’t working for you...ethanol, or endotoxins. The most common method for checking both quantity and quality of your plasmid prep...spectrophotometry. Achieving the highest-quality DNA requires taking care at each step, but some purification kits make...secret that labs produce a lot of waste. If you are looking to reduce your lab’s environmental impact, check... and yes those purification columns we’ve been talking about!) are a significant source of waste in laboratories...

  8. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...protein AraC binds O and I1 sites upstream of pBad, blocking transcription. The addition of arabinose causes... cAMP and represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters... be compatible with your experimental system. Leakiness If you’re expressing a gene that may be toxic,...inducible promoters are also known for their low leakiness. Inducibility level How much do you want to induce... inducible promoters can vary a lot. If you’re looking for high inducibility, the Tet-On system may be...

  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...can leave them at room temperature when you’re working with them, but avoid freeze-thaw cycles, as they...bind to amine groups present in some conjugates, blocking the conjugation reaction. Many commercial antibodies...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...in your machine before you make your purchase. Picking a single fluorophore for an experiment is easy,...accurate read and reduce needed compensation.    Working with antibodies can get a bit complicated, but ...

  10. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...responsibilities. After my second postdoc, I realized that working in the wet lab wasn’t the right path for me. I ...style of a new, junior team member who had values, working styles, and skills that were quite different from...from them. I started at my previous company by taking on responsibilities such as mentoring a junior ...Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned...Resources  Resources for aspiring managers Books: The Making of a Manager by Julie Zhuo Radical Candor by Kim...

  11. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...toolbox openUC2 are trying to overcome this hurdle by taking these limitations into account in the development...software (left) vs. open hardware (right).    Taking this all together, the essential ingredients of...newly developed techniques are the key enablers for making assemblies open-source. The sharing of digital ...produced quickly with the printer, act here as a kind of "teleporter," since a part developed in Germany...way of communicating in science brings stronger networking across laboratory and disciplinary boundaries...

  12. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...channel just for asking people if they can write or review things and another for asking specific science...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and...review to catch those pesky typos, and hit publish. Making content is an iterative process. We are constantly...

  13. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ... in Your Working Day" video from Science Media Savvy At this point, you might be be thinking social media...first blog post out of boredom. I had just started working in a new lab and I was the only person in the lab...first post so I signed up to write a second. While working on this post, I was offered and accepted a position...on the Addgene blog Read about online social networking for scientists Learn why scientists should be...

  14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... the mice to increase their nose-poking behavior. Strong nose-poking behavior is exactly what was seen...a channelrhodopsin, such as cell localization, kinetics, and spectral properties, must be optimized simultaneously...photocurrent strength, wavelength sensitivity, and off-kinetics.   This model was then used to predict which channelrhodopsins...photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s predictions.  Three...

  15. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...digest/ligate methods and picking the right one can save you time if you’re working at speed or money if budget...you can explore to maximize the use of both when making plasmids. Use repositories Repositories such as...free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster to order...experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try constructing several...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... unique cutting features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering...same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally...editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting single...use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting single stranded...single base pair edits, these enzymes are worth taking a look at. Choosing the right Cas can really put...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...on everything, including your skin. Here are some general tips for working with RNA, even if you are using...basics of isolating RNA without a kit. Tips for working with RNA (whether you are using a kit or not) Although..., 2006).  View our protocol for RNA extraction! Making the simple protocol even more foolproof (still ...still without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

  18. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... it’s monumentally difficult without software. Tracking lab procedures also gives you access to hard data...repeated. We also now have a good interface to begin tracking analytics data for lab procedures. With this data...are distinct from their contents. You should be tracking both, you should easily be able to move contents...less time getting up and running and more time working on interesting biotechnology code.There are valid...reasons to choose a non-Python stack, but if you're working with scientists it's unlikely they will have exposure...

  19. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... There are currently 17 sites around the USA undertaking BEST programs, including local schools in Boston...contributing to the NIH’s Biomedical Research Workforce Working Group. Along with her participation in this panel...Pubmed Central PMCID: PMC3787295. 6. Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901...PMC4185419. 7. NIH 2012. Biomedical research workforce working group report. http://acd.od.nih.gov/biomedical_...in Science in Our Career Posts! Get Advice on Networking at Scientific Meetings from Addgene Executive...

  20. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...edit and homology to the genomic DNA locus. After nicking by Cas9, the homologous pegRNA sequence hybridizes..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...previously used when they developed base editing. By nicking the non-edited strand, they can cause the cell ...efficiencies 2-3-fold. However, the process of double nicking increases indel formation slightly. Designing the...the edited strand, as in the PE3b system, guides nicking of the unedited strand only after editing has occurred... ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand Favor the edited strand...
Showing: 221 - 240 of 791 results