We narrowed to 329 results for: NUC

1. What's New in CRISPR - Spring 2019

   Type

   Blog Post

   Published

   March 21, 2019, 12:47 p.m.

   ...for human genome editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b...

2. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   Published

   June 17, 2014, 7:57 p.m.

   ...technology, the ability to direct enzymes – from nucleases to transcription factors – to specific sequences...

3. Let There Be LITE Plasmids

   Type

   Blog Post

   Published

   Nov. 21, 2013, 3:56 p.m.

   ...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

4. Editor's Choice, August 2016

   Type

   Blog Post

   Published

   Sept. 2, 2016, 1:32 p.m.

   ...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

5. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

   Type

   Blog Post

   Published

   May 17, 2016, 2:30 p.m.

   ...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs...

6. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

   Type

   Blog Post

   Published

   June 8, 2023, 1:15 p.m.

   ...microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...

7. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   Published

   May 12, 2015, 1:32 p.m.

   ...Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/...

8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

   Type

   Blog Post

   Published

   July 29, 2014, 6:16 p.m.

   ...Baird says. From F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently...

9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

   Type

   Blog Post

   Published

   Sept. 12, 2023, 1:15 p.m.

   ... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...

10. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    Published

    July 27, 2021, 1:15 p.m.

    ...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...

11. Fluorescent Proteins 101: Fluorescent Biosensors

    Type

    Blog Post

    Published

    May 18, 2017, 2:30 p.m.

    ... a particular cellular compartment such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole...

12. Sensing Neuronal Dopamine

    Type

    Blog Post

    Published

    July 30, 2019, 12:59 p.m.

    ...Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine...

13. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type

    Blog Post

    Published

    Feb. 1, 2018, 3:07 p.m.

    ...region with a high amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing...

14. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...

15. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...disease-causing point mutations. They allow single-nucleotide modifications to edit DNA without requiring a...

16. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...

17. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    Published

    Sept. 18, 2018, 1:33 p.m.

    ... find the ACE reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

18. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...researchers used the HA frankenbody to successfully track nuclei in a developing zebrafish embryo over an 80 minute...

19. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...chemical fluorophores for your next experiment. Nucleic acid based machines Recombinase-based State Machines...

20. Using Addgene's New Viral Service for Your Research

    Type

    Blog Post

    Published

    Nov. 17, 2016, 3:30 p.m.

    ...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...