We narrowed to 279 results for: Plat;

...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...

...expression consists of transfecting cells with your DNA template of choice and allowing these cells to transcribe...

... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...

...cleavage”. Jacob computationally added the non-template strand to the Jinek structure so that the resulting...

... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

...’s best to turn to an established social media platform. During the conference If the conference has a...

... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...

...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...

...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....

...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...

...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...

...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...