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We narrowed to 26 results for: Plat;

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Showing: 1 - 20 of 26 results

  1. CRISPR Guide

    Type
    Collection
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 — improved PE2/PE3 to inhibit mismatch repair...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9...luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to...expression vectors for cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS...contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-...for genome editing in the brain Using Cas9 mice Platt et al. demonstrated in vivo genome editing in the...knockin mice for genome editing and cancer modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR...

  3. Immunology Research Plasmids and Resources

    Type
    Collection
    ...JULF2, KPG_004, NOP9 PF4 platelet factor 4 CXCL4, MGC138298, SCYB4 PF4V1 platelet factor 4 variant 1 CXCL4L1... pro-platelet basic protein-like 1 TGB2 PROK2 prokineticin 2 BV8, KAL4, MIT1, PK2 PTAFR platelet-activating...PRSS26 PDGFA platelet-derived growth factor alpha polypeptide PDGF-A, PDGF1 PDGFB platelet-derived growth..., SSV, c-sis PDGFC platelet derived growth factor C FALLOTEIN, SCDGF PDGFD platelet derived growth factor...PENK proenkephalin - PF4 platelet factor 4 CXCL4, MGC138298, SCYB4 PF4V1 platelet factor 4 variant 1 CXCL4L1...THBGB, THBGB1 PPBPL1 pro-platelet basic protein-like 1 TGB2 PPBPL2 pro-platelet basic protein-like 2 SPBPBP... plexin D1 KIAA0620, MGC75353, PLEXD1 PPBP pro-platelet basic protein (chemokine (C-X-C motif) ligand ...

  4. Plan Your Experiment

    Type
    Collection
    ... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle on your gRNA, ...Editing : pegRNAs must serve as both a guide and a template. In general, edits must be downstream (3’) of ...edit site yield higher editing efficiencies. The template sequences can also have a large impact on editing... desired edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired...

  5. Antibody Guide

    Type
    Collection
    ... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... a target gene. On the other hand, if a repair template with homology arms was supplied, the break could...could be repaired according to this template, allowing for precise gene editing. These edits can include...work to develop a highly specific, programmable platform well-suited for various biological and translational...enabling access to multiple plasmids used for various platform applications. For more information on CRISPR, ...

  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole...BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T)-BGHpA AiP1786... AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m... AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA AiP13038 ...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...101 post for more information about HDR/repair template design. How to use CRISPR to tag your gene of ...encodes both a guide RNA transcript and a repair template for an individual tagging event. The kit contains...plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP...

  10. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...specific genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  11. TALEN Engineering

    Type
    Collection
    ...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...

  12. Ras Pathway

    Type
    Collection
    ...Cdc42/Rac)-activated kinase PDGFR PDGFRA PDGFRB Platelet derived growth factor receptor PDPK1 3-phosphoinositide...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum TALENs assembled by Golden Gate. Validated...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...human iPSC-derived neurons with a CRISPRi-based platform . (Tian et al. Neuron. 2019) See More AAV Viral...high-volume hybridoma sequencing on the NeuroMabSeq platform. (Mitchell et al. Sci Rep. 2023) See More Fluorescent...

  15. KLF Research Plasmids

    Type
    Collection
    ...Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi Michael Ruppert...

  18. Synthetic Biology - Overview

    Type
    Collection
    ...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

  19. Institute for Protein Innovation

    Type
    Collection
    ... in the collection have been developed using a platform (Link opens in a new window) based on yeast display...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...binding site (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the ...
Showing: 1 - 20 of 26 results