We narrowed to 289 results for: Pol;

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

2. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   Published

   Nov. 3, 2020, 2:15 p.m.

   ... have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure 2: ChRgers...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

5. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

6. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

7. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

8. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

9. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

10. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

12. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

13. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

15. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

16. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....

17. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

20. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...