We narrowed to 290 results for: Pol;

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

2. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

3. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

5. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

6. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

8. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

10. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

11. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

12. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

13. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

15. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

18. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

20. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....