We narrowed to 347 results for: SAM-2

...elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you choose ... last step will influence the stability of your sample over time and determine which experiments you can... impact the time you will be able to store your sample without having substantial degradation and which... DNA, it will limit what you can do with these samples. This is because divalent cations are essential...reaction, an effective possibility is to dilute your sample in water to reduce the EDTA concentration. Alternatively...

...prompt you to log in or create an account.  Figure 2: The new deposit materials bar New menu Most common...Materials”. The “Search Repository” function is our same search bar. And if you’re in the mood to browse ...

...PMID: 24336571. PubMed Central PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang...available for a little over a year and a half, a small sampling of the research it has already been used for includes...

... unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring. Hiking all...highly complex hot spring sample. The sample was separated into smaller samples of 5-10 cells each for sequencing...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ...biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles...from these extreme environments. Gearing up for sample collection at the hot springs My husband, Paul ...post doc in the Quake Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the... and two deep cycle batteries for power so the samples could be kept frozen after collection. Figure...

...Information Science and Technology 60.4 (2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research...Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools...amount of interdisciplinary research while at the same time increasing interdisciplinary citations in their...

...PMID: 25386923. PubMed Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S...N- and C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...

...bringing in environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma ...commonly used for every cell culture. Reusing the same bottle of serum again and again for each subculture... changed for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar...

...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...carrying sample-specific barcodes. Rather than continuing to process 100s to 1000s of samples individually...projects in many labs. However, the high costs of sample preparation for high-throughput next generation...technology overview For any NGS experiment, the DNA sample being sequenced must be sheared into smaller fragments...workflows are limited by costly library prep, as each sample requires an individual library. The plexWell™ workflow...library prep”, in which a relatively large number of samples are subjected to a DNA-barcoding step that uniquely...labels DNA from each well of a 96-well plate. Then, samples from several plates are pooled together to create...

...that underlie a particular phenotype. Figure 2: Schematic of CRISPR knockout screening with a pooled... each is a collection of plasmids that share the same backbone and differ only in a small region. This... the collection all the time! We’ll serve up a sampling of some of the most popular and give you a tour...tour of what these libraries are capable of at the same time. Barcoding libraries Just as physical barcodes...BioRender.com. The CellTag libraries from the Samantha Morris lab use a combinatorial barcoding approach...

...access to the results of federally-funded research. 2)      In June, the White House honored 13 “Champions...does about the state of peer review. 8)     That same issue of Science also included an online survey ...

...electrophoresis, you only need 1-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA...absorbance. Then you measure the absorbance of the DNA sample.  These absorbance measures give you an idea of...especially when you expect low concentrations in your samples and are often used to quantify DNA for next generation...fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity and their corresponding...That way, you can compare the fluorescence of your sample against this curve to quantify your DNA prep. Though...yourself as many fluorometers will calculate your sample concentration for you. Agarose gel electrophoresis...concentration of each band in the ladder. You then run samples of your DNA at different dilutions and quantify...

...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity...GUS staining or are not suited to studying large samples. RUBY-Red But recently, something new has been ...consisting of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and...transformation next to an untransformed seed from the same plant beginning to germinate. (Top right, second...

...double strand break), increasing editing efficiencies 2-3-fold. However, the process of double nicking increases... editing as efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime...using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025...likely because both innovations aim to solve the same problem. Both approaches are effective, but users...

...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic...microtubule-severing protein, Katanin is of course named for the samurai sword called a katana. Opto-katanin consists of...

...citizens enter into in order to protect their rights (2). Less formally, governments are the organizations...science education and literacy that this changes.  2) Lumping all scientists (and consequently their results...politician Rush Holt said, government must have the same fundamental attribute that enables science: “humility...

...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding... However, the acceptor doesn’t need to go to the same exact location as the donor. As long as some of ...targeted nearby (such as a neighboring house on the same street as the donor), then FRET measurements are...Ebright, 2001). These Cy/NTA conjugates work the same way as NTA-sepharose beads used for protein purification... binding sites are adjacent to each other on the same protein and a negative control experiment conducted...

...that you can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on...you identify when others have added or removed samples. Once you've taken some time to get organized, ...

...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to...validation screen using selected gRNAs extracted from the same or another library. These different approaches could...Listed you can also easily extract gRNAs for the same gene from several libraries, and identify those ...

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...researcher doing some experiments in Brazil can have the same issues as one doing experiments in France. We can...