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Addgene

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We narrowed to 511 results for: Set

Showing: 221 - 240 of 511 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't...

  8. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase...

  11. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... base editing and prime editing. How do you then set up an MBE system that contains a double-win for both...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... Yingxiao Wang lab.  The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...that is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...cell types of interest. By correlating your data sets, you can increase confidence that the labeling observed...available. Various proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g...

  19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

  20. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...
Showing: 221 - 240 of 511 results