...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...s41587-021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...

...transcription start site. In STARR-seq, randomly sheared genomic libraries are cloned downstream of a minimal...simple and complex DNA constructs Have you ever wished you could design a gene knockdown or overexpression...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository...

...to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com. Figure... Alyssa Shepard...

...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex...

...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous...

...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or ...some of these plastics are high grade and can be washed and reused. This might sound risky in terms of ...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh...

... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ...

...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

...irreversible protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences...Proceedings of the National Academy of Sciences paper [published online on March 17] should cover it. Addgene: ...

...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the...

... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack...

...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer...

...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, ...

...video of her singing about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich...UriAlonWeizmann, here my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive....

... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to...

...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe...can figure out what they are necessary for. We published our first paper on optogenetic tools for neuroscience...