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We narrowed to 608 results for: Sis;

Showing: 221 - 240 of 608 results

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...categories: probes and editors. A probe array may consist of at least two sensor modules targeting a specific... which can be easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H... EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...graduate students who work as teaching or research assistants to join unions. The topic of unionization continues...of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University of Massachusetts...PhD - Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...gene networks for the detection of antibiotic resistance and strain-specific diagnostics for the Ebola...Pardee and Alexander Green! Keith Pardee is an Assistant professor in the Leslie Dan Faculty of Pharmacy...the environment.     Alexander A. Green is an Assistant professor at the Biodesign Center for Molecular...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...silencing of self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based...address the growing clinical problem of antibiotic resistant bacteria. See the picture to the left for his ... vectors are engineered to replace the drug resistantance genes in the bacteria thus making the bacteria...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients lives. References...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...one.   SMASh Small molecule-assisted shutoff (SMASh) functions on the basis of both a degron and a viral...Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, ...several groups have designed modular toolkits to assist with tagging genomic loci in other model systems...including mammalian cells. CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung Lab...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...refers to the second step of this process: the synthesis of proteins from mRNA and the addition of post-translational...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of...the infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru K (2014...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

  14. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...results you need.  Whichever method you choose, resist the temptation to over interpret your results: .... PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler...amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):...

  15. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...cloning rings to isolate colonies of antibiotic-resistant cells; during the time required to grease, place... below) is simple: After obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual...specific wells from the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have...

  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...to 1:32. Each individual step of the series is consistent at 1:2. When preparing a dilution series, use...non-linear and you should use a different method of analysis. Different fields and assays have varying criteria...run.  When setting up your dilution series, use consistent dilution factors, change pipette tips regularly...

  17. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...the flanks of each ligation product, which is the basis for the repeatability of the method. Note that the... 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of...Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies ...

  18. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...the detection of rare sequences and single cell analysis. It is also being used in the detection of microbes... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes & infections 7.1 (2018): 1...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated...SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA targeting by a transposon-encoded CRISPR–...
Showing: 221 - 240 of 608 results