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We narrowed to 630 results for: Son

Showing: 221 - 240 of 630 results

  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides a selection...households by observing proper cleanliness and personal hygiene standards. Handwashing is an essential...spread of antibiotic resistant bacteria from one person to the next. For more information and tips on preventing...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable effect...and can be easily programmed for new genes. In comparison with other large insert techniques like HITI ... Rachel Leeson...

  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...screening! This blog was written by Addgenie Hannah Dotson.  Resources and references References Genome-...protocols.  References McDade JR, Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled...  Hannah Dotson...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...lack of documentation and high turn-over of lab personnel. If you are unsure of the background of the lines... such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections ..., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma infection significantly alters...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...different projects, you might not be able to make any reasonable progress on any one of them. That’s why, as I...recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do lists. Because...Bored and Brilliant” for learning more fantastic reasons to remove yourself from distractions every once...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ... a fluorophore (Figure 1).   Figure 1: Comparison between how direct immunofluorescence and indirect... will focus on this technique here. One of the reasons that indirect IF is more common is that secondary...primary, resulting in signal amplification. Another reason is cost effectiveness. Generating and validating...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water...  Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M....Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...be quantified by the strength of the signal in comparison to a control sample. Southern blotting applications... sequencing of many samples at once for later comparison. These next-generation sequencing techniques ...Sanger, Frederick, Steven Nicklen, and Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...being mentally challenged. For these and other reasons, I started  looking for other career options after...government loans to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry...or another country of the Commonwealth. Another reason why I chose Australia was because it is an English...

  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...cross country boundaries for both professional and personal development, is essential to excelling in the ...adapt their mentoring styles to the mentee’s personality and professional needs at any time. In its Pilot...advisor. For their own part, mentors receive immense personal gratification as they witness their student’s ...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...that contains sequence complementary to the dsRNA (Wilson and Doudna, 2013). RNAi consists of two non-exclusive..., Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential...:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA...

  12. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...difficult to trace. These include the laboratory personnel, the serum, the cell culture media, water baths...your cell cultures because you are not the first person to face this kind of problem. Educate others when...Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... were suitable for our needs for the following reasons: Safety: Since AAVs are nonpathogenic, we are...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...applications, we may also be able to use these comparisons to enhance our understanding of receptor physiology...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ... gRNA plasmids on our website. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

  15. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

  16. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

  19. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...how Nextflow helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our...  Rachel Leeson...

  20. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...
Showing: 221 - 240 of 630 results