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Showing: 221 - 240 of 482 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other...Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids Should I...

  2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Dolan, F100Research, 2016). These events have also started progress towards solutions. The 2015 Boston FoR...problems facing postdocs and graduate students, and started to define solutions but this year, together with...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...101 eBook to get all the background you need to start working with plasmids! What are termination and ...Addgene.org Browse our Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid Collections...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...relatively quick. After transfection, HEK-293T cells start producing virus within 24 to 48 hours. Harvesting...can even produce a P3 virus! Time Sf9 cells will start producing virus within a day or two, but unlike ...

  7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...definitive, but visual identification is a good place to start before taking on more time consuming and expensive...use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used codon optimization programs...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...directed to a target upstream of the transcriptional start site (TSS) of a gene of interest, it induces potent...directed to a site downstream of the transcriptional start site of a gene of interest, Cas9 forms a steric ...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...of common E. coli strains. Increase the volume Starting with a larger culture volume can improve plasmid...serve to increase cell density and plasmid yield. Start with a single, fresh colony Sub-culturing directly...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...in the example above that the bread crumbs trail starts in the browse page, continues through the depositing...the plasmid in your experiments: Backbone: The starting vector that the depositing lab modified to create...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...the secondary nanobody toolbox Are you ready to start using nanobodies for your own research? Great! Pleiner...Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...curriculum. It’s through the PTA that I recently started coordinating the annual Math & Science Night, a...scientist. I gave a timeline of my science life, starting back to Mr. Kelley, my 7th grade biology teacher...

  13. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...specialized equipment and/or expertise from the start, or if you can begin simply and build up to more...potential of your preferred platform before you start can save a lot of trouble in the long run.  Selecting...

  14. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...depend on the host cell’s initiation proteins to start their own replication. In general, these plasmids...binding of the low-affinity versions is required to start the replication process. When enough copies of ATP-DnaA...

  16. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...primer pair. Ct values vary based on the amount of starting material in the PCR because the sample must fall..., which is a factor in AAV titration since the starting template is the purified viral vector solution...

  17. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... Again, attending one of these fairs is a great start, but you need to do more than just show up. Do your... particular employer in mind, you may consider starting with indeed.com or monster.com, where you’ll find...

  18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...homology to the crRNA or presence of a PFS. This is in stark contrast to Cas9, which requires that each DNA target... we know about its structure and function? For starters, Cas13a can be used to bind and cleave target ...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...may be preferentially charged during amino acid starvation thus resulting in higher production of amino ...charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified from patient samples and can...applications. The company Mammoth Biosciences has been started based on DETECTR technology with the mission to...
Showing: 221 - 240 of 482 results