We narrowed to 323 results for: Stil

1. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering...

2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...

3. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...

4. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...they retain their intracellular interactions and still set off the same pathways, giving rise to the same...

5. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...cycle once inside the host. While the virus can still enter (and thus infect) cells, this safety feature...

6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...you can make big changes in your career path and still find comfort and happiness there. Read all of the...

8. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...targeting the same cell class in all three species are still greater than random chance. The chances of a promoter...

9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...Table 1). Additionally, hiPSCs and mouse BM cells still behaved like iPSCs and BM cells after Nanoblade ...

10. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...mechanisms behind the immune defects in CDG patients are still poorly studied and understood.  In line with this...

11. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...use of this system. New and improved systems are still being developed. A detailed protocol for production...

12. Rabies and Neuronal Tracing

    Type

    Blog Post

    Published

    May 29, 2018, 1:51 p.m.

    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...

13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

14. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...a minute, when it is only warm but not hot- and still liquid- it is rapidly poured onto the middle of ...

15. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    .... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...

16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...limited to restriction enzymes - although many labs still love using them! With so many technologies currently...

17. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...these systems, though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors...

18. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

19. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...that they were having a positive experience, I was still incredibly nervous about leaving academia. A lot...

20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...