...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1...

...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...

... and Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1...

...substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent...

...years from symptom onset. Disease mechanisms are still poorly understood but several genes and mapped loci...

...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

...For small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size...

...Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available) Based...

..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of...

...Equipment Water Bath 10% bleach or 70% ethanol Distilled water Disinfectant Thermometer Water bath weights...the edge of the slide. Fill the water bath with distilled water, not tap water, to prevent salts from accumulating...water in it from previous uses. However, you will still need to regularly clean out the water bath even ...

... with the recipient plasmid's MCS. However, you still need to avoid restriction enzymes that cut within...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

...entire tube is dried but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water...

...solution by dissolving 40g of LB powder in 1L of distilled water. Autoclave and cool to 55°C. Add 1mL of ...of puromycin (Sigma-Aldrich catalog #P8833) in distilled water. Sterilize by passing through a 0.22 μm ...

...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...

...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...

...time by shortening or skipping many steps and will still get enough colonies for your next step. Remember...

.... You can take entire courses on microscopy and still have more to learn, so, for this protocol, we'll...

...20 min) but check for the presence of particles still in solution. Continue to stir until all particles...

...untransduced cells, resulting in a more homogenous (but still polyclonal) cell population. Depending on the transducibility...

...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...