Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 546 results for: TIL;

Showing: 221 - 240 of 546 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...expression of the gene it follows. For increased versatility, it would be advantageous to adapt PITCh to insert...-independent knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use...

  2. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...develop formal guidelines to answer this question. Until then, using multiple confinement strategies is strongly...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...and gene therapy AAVs for genome editing AAV: A versatile tool for gene expression in mammals Resources...

  5. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... and plan for the future of the team. All while still paying bills and having a life outside my job. The... and fail to identify it as a personal strength until it is identified by another person. Similar reasoning...

  6. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...period of time. Though climate change certainly still is and will continue to be a “critical consequence...current period of rapid flux, the chapter might still feel slightly dated by next week.   A stepping stone...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...

  9. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as ...elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...and well-documented history of racial bias, which still impacts and influences current research. Understanding...five-team response to address this issue, but it still points to a far larger and more complex problem ...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ...

  12. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...reporter gene. The polymerase will elongate the mRNA until it reaches the gene end signal, where it will terminate...reconstitute a functional viral polymerase complex and utilize the minigenome as a template. Thus, it is important...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...imagine, it keeps all of us quite busy. Though there still isn't time for goofing off, the company culture ... the lab was such a master drywall hanger?  4. Utilize your office space too Balance point number 3 with...

  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Sanger sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one AAV, Cas9 must...Cas9. Unfortunately, certain drawbacks limit the utility of these systems: St1Cas9 requires a very specific...

  17. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transferred into the plant. In the laboratory we can utilize this natural process by replacing the genes on ...genes and speeding up research. Certainly, there is still more to transient leaf transformations, but we hope...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...known as channels. Since a Nextflow process can utilize any scripting language that can be executed on ...processing of our sequencing data. However, our work still wasn’t complete. In order for our QC team to smoothly...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...far messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen...discovery, and food safety.  Part of the assay’s versatility lies in the user’s ability to modify the protocol...
Showing: 221 - 240 of 546 results