...different mutations found in leukemia, the Lowe lab was able to identify a set of mutations that made ...Lowe’s lab utilized mice to study how different mutations in leukemia impact different treatment regimens...by a specific gene. For example, several genes mutated in human cancers have a homolog involved in yeast... yeast: A model organism for studying somatic mutations and cancer." Nature Education 1.1 (2008): 183....

...genetically encoded fluorescent probes. One of the limitations is the size of GFP: ~240 amino acids or about...supplementary figure C). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (...the appearance of OSER whorls. This assay is quantitative and is currently the standard for assessing ...straight fusions, without any linker. When both orientations are available, a direct comparison of these ...PMCID: PMC3324619. Cranfill, Paula J., et al. "Quantitative assessment of fluorescent proteins." Nature ...

...in cells, they have various disadvantages and limitations – for example, microtubule-destabilizing drugs...damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the... et al., 2022), that overcomes many of those limitations (Figure 1). Figure 1: Opto-katanin induces fast...reversible microtubule severing and has very low dark-state activity and toxicity. All of that means this light-powered...moderately-enhanced 10x and 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and...

...conditions. As a facultative aerobe, it can thrive with or without oxygen. Additionally, its status as a Biosafety...used as a model organism for over 60 years with a status as the most studied prokaryotic organism. With ... biofilms, and much more. Because of it’s BSL-1 status, E. coli can be used as a model to relate these...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

...also use different cytoskeletal machineries to facilitate transport: retrograde transport relies on dynein...2014). It is currently unclear which proteins facilitate transport of neuronal tracing compounds and this...-Gold (Schmued and Fallon, 1986) are used. To facilitate cellular uptake of these dyes in vivo, microinjection...a negative impact on cellular health. Another limitation of traditional tracing compounds is that their...rAAV2-retro’s altered amino acid sequence might facilitate interaction with uptake or intracellular transport...mechanisms of intracellular transport of AAV could facilitate the development of more powerful AAV serotypes...

...genome and are inefficient at introducing point mutations. In 2016, the researchers at the Broad Institute... envisioned a new strategy to introduce point mutations in the genome. They fused a catalytically impaired...a tRNA deaminase enzyme, TadA) to induce a A→G mutation at the target genomic loci. Together, CBE and ...the prime editor can install all possible point mutations, delete short stretches of DNA and even insert...doi.org/10.1038/nprot.2013.143 Seligman LM (2002) Mutations altering the cleavage specificity of a homing ...

...need representatives and policymakers who not only believe in the separation of church and state, but ...Institute, most women of reproductive age live in a state with at least two abortion restrictions that conflict...are that we, as a society, need to: 1) use more citations for out data so our sources are readily available...the Vancouver SciCATs. Follow her on twitter @Point_Mutation, and @SciCATSyvr. Additional Resources on ...

... aside for the event. As guests arrived, some gravitated towards the sushi and drinks, while others headed...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...

...final version of the document. Set and manage expectations When the researchers involved in a project are... crucial that everyone meets early on to set expectations for the output that will come from the collaboration...should then hold each other accountable to these expectations once they’re set by checking in about them at...speed up the future directions of the project. The state of research is constantly shifting, and long-distance...

... does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations and makes ...graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq... binding patterns that overcomes some of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage...

...several struggles with these techniques: unwanted mutations due to excessive PCR cycles or low fidelity Taq...purify and sequence the plasmid to confirm no mutations were introduced during PCR or any subsequent steps...fidelity DNA polymerases, I have never had any mutations introduced into my inserts. However, with any ...technique there is a chance of introducing an unwanted mutation. Tips for high efficiency cloning Now that you...

... used SMRT sequencing, TA-based ligation and tagmentation-based methods for AAV genome sequencing (Tai...self-complementary AAVs and the TA-based ligation and tagmentation preparation approaches described previously ...single-stranded and both TA-based ligation and tagmentation require a double-stranded template.  Using viral...the mix to serve as effective substrates for tagmentation-based library preparation procedures. This finding...

...single molecules and newly formed proteins.  Limitations with GFP in imaging  While GFP has been an immensely...to study proteins in vivo, it does have some limitations. GFP has long protein maturation times which ...antibody based probes a solution? To address these limitations, scientists have co-opted antibodies for tagging... the National Academy of Sciences of the United States of America, 69(9), 2659–2662. PubMed PMID:4560694...

...however, has inherent limitations because it turns a dynamic process into a static measurement and it provides... acidic environments at 586 nm. Regardless of excitation wavelength, Keima emits at 620 nm. The ratio ... Noda, Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods...

...choice while bypassing the intermediate pluripotent state. Though a valuable tool for regenerative medicine...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In this lineage...reprogramming. MettI7a1 encodes an uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard...

...forming concatemers later. The "FS" stands for Fragmentation System and this is the most important part of...for library preparation using this kit is: Fragmentation and end repair Adapter Ligation Clean up/Size... the start of the workshop, NEB gave a brief presentation describing the technology. Then we split into...and network with other technicians/company representatives. I got to talk with a technician from Neon ...

... gRNA representation, produce high titer lentivirus from the library, ensure gRNA representation in the...technologies in plasmid form and are proud to have facilitated their fast adoption. However, in many cases the...screening-scale experiment achieving the gRNA representation recommended in the publications associated ...

...train and acquire the data you need on the new instrumentation. Bonus reasons Visiting gives you access to...This probably isn’t the lab’s first request for visitation if they have specialized skills and equipment...so that you can work together to ensure your expectations are reasonable, your reagents are onsite before...include, but is not limited to temporary housing, transportation, living expenses, and necessary reagents. Your...

... sketch on the board without leaving Zoom. The annotate function can also make the screen-sharing experience...top of the screen and every participant get to annotate things as they come up in their minds. The final...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...where all lab members talk no science. No data presentation will appear here, and only general chatter will...

...success. It is imperative that you resist the temptation to “just do it myself” or micromanage –you don...develop the web page. The team manager must then facilitate appropriate communication between members of ...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone..., meeting minutes and plan changes. Written documentation helps make sure everyone is on the same page...