...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...libraries makes them the ideal vector for the job. Therapeutic applications Vaccine development Attenuated and...

...computational processing with biological protocols and revolutionary physical approaches. However, with each new ...evaluating antibody staining, or counting cells are now routine, open-source software projects that actively engage...community to regularly replicate designs worldwide. Distributing hardware via globally established makerspace...

...sequences. Researchers have also used experimental evolution to develop Cas9s that have a broader PAM specificity... recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with...PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 to increase its specificity." Nature...

...connectivity patterns of these cells and the functional contribution to a variety of behaviors related to addiction...Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian”) is a postdoc...video-based high-throughput screen for directed evolution of biosensors based on their dynamic properties...

... plasmids and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting...

...sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this...misinterpretation was definitely happening. Our solution for this problem was the creation of an Addgene...sought buy-in from all of Addgene’s teams. This solution can easily be implemented in an academic lab setting...

...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable to engage...initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to control the...

...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through... shows that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers... both sexes ensures that the diagnostics and therapeutic interventions you develop represent the whole...

... interest. Endogenous gene tagging is an ideal solution because it allows for tagging and visualization...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...plasmid backbone for delivery to the cells. As a precautionary measure, it’s also good to make a few single...

...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... realization that, if we want people to keep contributing to science, it would be helpful for them to ...we forget that some of the terminology we use routinely isn’t common to non-scientists and may make your...

...studies are expected to identify new therapeutic targets/solutions and enable scientists to discover if...sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2 (1998):...

...dust since the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant...Therefore, researchers from over 100 scientific institutions have proposed a shift to recombinant DNA-based...

...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ...as a STAR answer: a setting, a conflict, and a resolution. Stories usually have a theme too, which is something...your morning coffee, if that’s part of your daily routine. Stay hydrated. You might have lunch on your schedule...

...Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing ...der Oost J, Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems. 5. Nat...

...focus on one such strategy--the FLEx switch--which utilizes recombination elements to turn off expression ...introduced, recombination can proceed either by first utilizing the loxP sites or the lox551 sites. Regardless.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

... area on the outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science... “we underestimate living things.” He further cautioned the distinction of “higher” and “lower” life forms...of years of common ancestry with worms before evolutionary divergence. Nikolaus Rajewsky from the Max Delbruck...

...and to become involved in developing tools and solutions to research problems. This movement calls itself...Minnesota has declared our makerspace an educational institution meaning that we can obtain known mutants at $7.00... school to merge his interests in biology and computing. In 2009, he founded a team at UVic to compete...

...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can...Furthermore, among those Cas9 orthologs that have been scrutinized, the majority have been shown to exhibit more...

...DNA constructs designed for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust...when a protein is expressed through the use of constitutive and inducible promoters. Inducible promoters...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...