...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...lab researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, ...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

...noticeable influence on the outcome and cause variability in the testing group. For example, if an experimenter...distracted) from the ones tested at the beginning. Variability source Standardization parameters Animal Strain...This time window between injection and testing will vary depending on whether it is an overexpression gene...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates and even clonal ...CRISPR community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...promoters, AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking...

...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...several excellent reviews have collated data from various papers to make the decision easier. 1. Müller, ... because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent...

...protocol [3] provides, in exquisite detail, the various steps required to produce your fiber optic implant...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

...movement and is part of a bigger movement in a variety of research sectors to provide open source technologies...engineers, and biologists working together on a variety of projects including the OpenWorm project. The...model, and the biologists can get involved a in variety of aspects from the molecular biology to learning...

...because different CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...

...biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression for experimental...protein tags and separated from contaminants using a variety of purification methods. Deciding which expression...expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

...versatility of Cas effectors allows for a wide variety of screens, including interference, activation,...using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

...the level of autofluorescence across various tissue types varies, it is critical to include an unstained... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

...volume = more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported...plasmid DNA yield. Different plasmids and strains will vary in their optimal growth conditions. For many high...cell density or by harvesting and miniprepping at various time points. Measuring the optical density of the...

...discovered that there are many ways to get a taste of a variety of careers while still working towards your degree...graduate office arranged opportunities to meet with various professionals, and there were multiple avenues ... Find Plasmids for Your Research Learn About A Variety of Different Fields on Our Collections Pages ...

...The VSD components have historically come from a variety of sources – voltage-gated potassium channels found...Chemigenetic Sensors These types of sensors can have a variety of different components, but all contain a FRET...upon host infection. Packaging viruses also have various serotypes – the surface proteins that confer affinity...

...Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms...seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety officer should... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...

...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...in therapeutic applications, the authors tested various dRanCas13b truncations that would allow packaging...

... new cutting edge science, researchers of all varieties must stay current with literature and emerging...clear that while several people across teams had various levels of experience with bioinformatics and HTS...welcome to ask any questions they have.  These variations are suited for different applications and should...

...one used in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of...serotypes are currently used in clinical trials, a variety of engineered ones have shown great promise in ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

...10% of DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...