Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 642 results for: Vars;

Showing: 221 - 240 of 642 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...

  3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

  5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...lab researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, ...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

  6. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...several excellent reviews have collated data from various papers to make the decision easier. 1. Müller, ... because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...promoters, AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking...

  8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...noticeable influence on the outcome and cause variability in the testing group. For example, if an experimenter...distracted) from the ones tested at the beginning. Variability source Standardization parameters Animal Strain...This time window between injection and testing will vary depending on whether it is an overexpression gene...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...protocol [3] provides, in exquisite detail, the various steps required to produce your fiber optic implant...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...movement and is part of a bigger movement in a variety of research sectors to provide open source technologies...engineers, and biologists working together on a variety of projects including the OpenWorm project. The...model, and the biologists can get involved a in variety of aspects from the molecular biology to learning...

  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... 2025). The team characterized and deposited a variety of constructs for visualizing cellular components...-based biosensor for cAMP dynamics. The mGold2 variants significantly enhanced photostability — 25-fold...with BioRender.com. When delivered by AAV to various brain regions in mice, ATLAS mediated anterograde...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...versatility of Cas effectors allows for a wide variety of screens, including interference, activation,...using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression for experimental...protein tags and separated from contaminants using a variety of purification methods. Deciding which expression...expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...the level of autofluorescence across various tissue types varies, it is critical to include an unstained... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

  16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...because different CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...The VSD components have historically come from a variety of sources – voltage-gated potassium channels found...Chemigenetic Sensors These types of sensors can have a variety of different components, but all contain a FRET...upon host infection. Packaging viruses also have various serotypes – the surface proteins that confer affinity...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...volume = more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported...plasmid DNA yield. Different plasmids and strains will vary in their optimal growth conditions. For many high...cell density or by harvesting and miniprepping at various time points. Measuring the optical density of the...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...discovered that there are many ways to get a taste of a variety of careers while still working towards your degree...graduate office arranged opportunities to meet with various professionals, and there were multiple avenues ... Find Plasmids for Your Research Learn About A Variety of Different Fields on Our Collections Pages ...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms...seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety officer should... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...
Showing: 221 - 240 of 642 results