We narrowed to 348 results for: abo.4

...Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental Microbiology... with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...

...https://doi.org/10.2174/1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

...experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

...points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer help tickets. Catch up ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

...30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn more about research...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using...using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org...

...Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...that can help you craft a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

... phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

...methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "Direct visualization of newly... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

...Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

...Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...PubMed PMID: 1495975. PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...activation and base editing applications.  Figure 4: Overview of CasMINI for genome engineering applications... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...in short term storage (think days to weeks) in the 4°C. If you’ll only be using it every few month or if...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...

...Improved signal-to-noise ratio (SNR). Figure 4: Calcium titration curves for OCaMP and various red... view of traces corresponding to the dashed box above. The number of action potential (AP) spiking events...

... Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84... Park et al., Science 2011 Aug 26;333(6046):1151-4. pOSIP and the Clonetegration Classic genetic ...assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the number of...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...pGreen-IIS based destination vectors. To learn more about the GreenGate cloning system, see the detailed plasmid...

...real-time PCR experiments. Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009...GAPDH. Biochem Biophys Res Commun. 2001 Mar 9;281(4):951-6. doi: 10.1006/bbrc.2001.4439. PMID: 11237753... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

...ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding affinity... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued presenting...silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in my head...doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week!  Nerves...attention? fear of messing up?–and use the three tips above to make a strategy that can help. Even something...speaking as a transferable skill The worst thing about doing public speaking as a scientist is that you...perfectly, you truly are a rock star.  The best thing about public speaking as a scientist is that you will ...increases visibility of your work, opens up collaboration and growth opportunities, and gives your entire...