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We narrowed to 340 results for: abo.4

Showing: 221 - 240 of 340 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer help tickets. Catch up ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn more about research...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using...using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org...

  4. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...that can help you craft a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "Direct visualization of newly... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  6. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ... phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  9. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...PubMed PMID: 1495975. PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...activation and base editing applications.  Figure 4: Overview of CasMINI for genome engineering applications... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...in short term storage (think days to weeks) in the 4°C. If you’ll only be using it every few month or if...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84... Park et al., Science 2011 Aug 26;333(6046):1151-4. pOSIP and the Clonetegration Classic genetic ...assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the number of...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...pGreen-IIS based destination vectors. To learn more about the GreenGate cloning system, see the detailed plasmid...

  14. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...real-time PCR experiments. Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009...GAPDH. Biochem Biophys Res Commun. 2001 Mar 9;281(4):951-6. doi: 10.1006/bbrc.2001.4439. PMID: 11237753... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued presenting...silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in my head...doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week!  Nerves...attention? fear of messing up?–and use the three tips above to make a strategy that can help. Even something...speaking as a transferable skill The worst thing about doing public speaking as a scientist is that you...perfectly, you truly are a rock star.  The best thing about public speaking as a scientist is that you will ...increases visibility of your work, opens up collaboration and growth opportunities, and gives your entire...

  16. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding affinity... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

  17. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Always ask for references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research...research.  It helps to spend some time thinking about what your goals are for the coaching relationship...with someone you connect with. Talk to the coach about their “style” and make sure this is a style that...coaching group is Still Point Coaching. One nice thing about a group is that sometimes this offers the option...

  18. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way to network...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...cardiomyopathy. An adult male with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen...August 2nd in Nature gives us even more to talk about, as it represents another step towards CRISPR germline...impossible with current tools, thus quieting some fears about the creation of “designer babies.” The differences...of individuals with varied backgrounds. Talking about this study with non-biologist friends Like me, you... Blog Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...to visualize low-abundance proteins. Figure 4: Overview of using Indirect ELISA to quantitatively...blog Learn about the advantages and disadvantages of using monoclonal antibodies. Read about other techniques... require two different antibodies. As mentioned above, it can also be used to characterize antibodies ...concentration of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins...
Showing: 221 - 240 of 340 results