We narrowed to 519 results for: ato
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TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....expression analysis to investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated...untranslated regions — on gene expression. The regulatory element is cloned upstream of the luciferase ...
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How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator. 6. Virtual interview prep Prepare for your... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m.... Expression Low Tropism for Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m...., one that is not food, not medicine, and not mandatory – people can decide for themselves if they want...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m.... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...your sequencing results back, you can use the chromatogram to spot whether any discrepancies between your... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... plasma membrane to be activated by the DREADD actuator after it has been administered. Although we found...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains contested...counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....this study laid the foundation for how different anatomical regions of the brain receive inputs and compute.... 5. Nassi JJ, Cepko, CL, Born RT, Beier KT. Neuroanatomy goes viral! Front Neuroanat. 2015 Jul 1;9:80... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...for other developmental models including flies, nematodes, and zebrafish Find plasmids for Mammalian RNAi...