We narrowed to 319 results for: bre

1. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ... tags influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific...

2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

   Type

   Blog Post

   Published

   Sept. 8, 2020, 2 p.m.

   ...study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

3. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin...

4. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...obvious explanation, but more often than not I had to break down exactly what I had done in order to identify...

5. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...transcriptase (RT), which induces two double-strand breaks and deletes the intervening sequence. As with other...

6. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

7. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible ...

8. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...function of guiding Cas9 to generate a targeted break. These advances in gRNA technology have enhanced...

9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ... different stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene...

10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    Published

    July 21, 2016, 2:30 p.m.

    ... cases of cardiovascular disease, 126,000 fewer breast cancers, and 145,000 more quality-adjusted life...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...proteins to form a tetramer. A double-strand DNA break occurs within the core spacer of each loxP site ...

12. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...eugenics and research misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the ...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...stress level, origin (vendor or in-house mouse breeding colony) Environment Olfaction, temperature, ...

14. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...dreamed of becoming a doctor. After taking a year break in between undergraduate school and studying for...

15. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...modifications). Ready to get started?   Figure 1: Break free from the ball and chain!   Epigenetics...

16. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field and the greater...

17. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 ...

18. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https...

19. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...coli is safe to use in the lab but also causes outbreaks of food poisoning, the difference is virulence...

20. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ... the Fragmid system, the Fragmid website, which breaks the design process down into four easy steps, provides...