...blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid pages We’ve given...but with far fewer off-target effects! This characteristic probably explains why pC016 - LwCas13a guide...RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue for temporary... Expanding CRISPR Beyond SpCas9 Day 8: Repair mechanisms: CRISPR 101: Non-homologous End Joining and PITCHing...podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening...

...take over. I like writing and science, so I took a chance.” It’s been six years since Hans took that gamble...science talk). I do illustrations for summarizing mechanisms and applications that are relevant to things ...favorite thing to work on. “It’s one of the most challenging [parts of my job], so making it a success feels...colors draw people in. Drawing a picture of a mechanism that also looks nice is a great way to bring people...posts. And make sure to subscribe to the Career Channel of the blog so you don’t miss the next two posts...

...conformational change in the GPCR, leading to the activation of the Gα subunit. The activated Gα exchanges bound...constructs, localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering...activation by drugs to further understand their mechanism. These plasmids are available as a collection,...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

...Mask: How Leaders Spark and Sustain Change, by Peter Fuda and Richard Badham Leadership Insights for Engineers...Stages of Your Career: Doer, Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers... are many good resources on the skills and characteristics of successful leaders. Below I've listed a ...Leadership (Articles on Leadership, also can be purchased individually) ...

...systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...how much more there is left to do with the characterization and implementation of this system. Monday ...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only... are taking the "genetic code" to a new level, changing it from a puzzle to decrypt to a tool to be harnessed... tools – both deposited and distributed – have changed so dramatically in the past 4 years. I kept busy...

...retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power so the samples... voltmeter on top of the battery box to monitor charge status and a disconnect switch between the battery...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples... Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S rRNA gene sequencing which...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 ...

...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright...mutagenesis approach and found that specific amino acid changes favored blue-shifting more than others. During ...Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging."...blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out...

...master’s thesis in science communication, I had the chance to carry out a comprehensive review of the literature...findings relevant to my background (neuroimaging, biomechanics, neuropsychology) and from areas in which I ... and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing effort...display in our building, each day brushing past and chatting with individuals whose research ranged from wildlife...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

...researchers working on rare diseases, offering them the chance to win some of the latest tools and technologies...They now have some theories on the underlying mechanism, involving the primary cilia. They’ll put a variety...number of stressors, including emotional stress or changes in temperature.  “The disease was first described...a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers...syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called...

...was researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most... choices we make, my job choices were mostly by chance. Luckily, around the time I decided to look for...have been their target market for many years and purchased their products, so you have some of the best ideas...started with the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled...post-doc a Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

... to a channel number based on intensity. Higher intensity events are assigned to higher channels. In a...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...of the protein. In some cases this can lead to changes or a complete loss of protein function. In addition...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...stream oscillates to generate droplets that are charged as they pass through a metal deflection plate. ...

...Fluorophore detection Live APC-Cy7 channel eGFP FITC channel CD45 BV421   Figure...of fluorophores from Figure 1. For example, the chart tells us that PE and PE-Cy7 are "very bright" when...conjugated to Cy7, however, (APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that ...fluorophore, we have to tell the cytometer which channel to use to detect eGFP. As you saw in Figure 4, ...overlaps with FITC, hence reading out the FITC channel in the cytometer's software (without using the ...

...above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation ...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as...reliably locate your injection site. We recently purchased this model ( no longer available through Kopf)...editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter 1:Unit 1 20 1-18. doi: 10.1002/0471142301.ns0120s59...PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection...

...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging period...Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16,...give back by supporting local businesses (ex: purchasing a gift card to use later or ordering take-out...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

...research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen...her group are interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance ... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... investigates the molecular and cellular etiopathomechanisms of ischemic stroke, the most common type ... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury....

...constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the transduced...is to increase the AAV concentration. In this flowchart, you can see the dwindling proportion of AAV particles...gets transported up the cell’s axon. While the mechanism for this function is not yet fully understood,...S9-S15. PubMed PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction...

...transcriptional changes. Using this road map, and what was known about previously characterized TFs, the Zhang...association of the uncharacterized TFs is a useful finding. Transcriptional program changes from this screen...can be used with other selection and screening mechanisms, although the unique barcodes for each TF help...

...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

... the materials in our catalog are very well-characterized. During this time, we’ve also created a variety...this, throughout the year we rolled out multiple changes to our website, including the recent homepage and... a look at the least visible part behind these changes: the backend technology that powers the new website...granularly customize the search engine, and a scoping mechanism that allows category-specific, narrow searches...among others. We also created a normalization mechanism to “collapse” variations and synonyms of the same...

...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....Optogenetic control of protein binding using light-switchable nanobodies." BioRxiv (2019): 739201.  Additional...