... plates. This prevents satellite colonies from forming while effectively selecting plasmid-containing ...cost-effective, timesaving for transformations.
Cons: Prone to satellite colony formation on agar plates, less ...in a plasmid. During the propagation and/or transformation process, some bacteria will either never receive... is nifty because the recovery period after transformation (the dreaded 60 minutes shaking at 37 degrees..., interchangeable with Amp, timesaving for transformations.
Con: Expensive.
Kanamycin
Kanamycin, or...selection gene. However, it does require a slower transformation recovery period than amp or carb (60 minutes...confers mammalian resistance to G418.
Con: Slow transformation
Spectinomycin/streptomycin
Spectinomycin...