We narrowed to 493 results for: multi

...That way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you... Multiple Authors...

... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

...electric current of ion channels in living cells. Multiple properties of a channelrhodopsin, such as cell... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

... kind of cellular computation, making possible multiple logic-gated steps. Beyond the lab, these systems...these tools are already deposited at Addgene in multiple formats: for use in different organisms, targeted...

...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...

...aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows...

... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...

...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...

...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...

...tiny DNA cutting ninjas that deliver CRISPR to multiple types of targets: primary cells, embryos, and ...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...

...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...

...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

...According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins... and CAA PAMs, they saw that xCas9 3.7 cleaves multiple PAMs at a higher efficiency than SpCas9: NGT (...

...editing efficiency of prime editing, whereby a multitude of configurations can be used to introduce specific...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...

..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM...

...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there...