We narrowed to 322 results for: nans

1. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ... he kindly took some time to address all your unanswered questions via text! (well, maybe not all of them...

2. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

3. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...

4. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes to the...

5. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog ...

6. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...minimized with high powered research designs. Unanticipated discoveries Finally, there are good reasons ...

7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

8. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive...

10. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...expression and largely defines the amount of recombinant protein that will be produced. Many common promoters...

11. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

12. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations...

13. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

14. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...

15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...against mCherry, and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution...

16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins should act as ...

17. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...that lacks genetic tools is the bee microbiome. Nancy Moran’s and Jeffrey Barrick’s labs at The University...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or...

19. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board...

20. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ... concentration of all the samples by gel or by nanodrop to assemble the  fragments based on their molar...