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Showing: 221 - 240 of 271 results

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Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

Published

Jan. 10, 2017, 3:37 p.m.

... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

Published

Nov. 5, 2024, 8:32 p.m.

... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

Published

June 25, 2024, 1:15 p.m.

...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

Published

Sept. 6, 2016, 2:30 p.m.

...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

Published

Sept. 24, 2015, 2:30 p.m.

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

Published

May 22, 2015, 4:58 p.m.

...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

Published

June 12, 2014, 3:30 p.m.

...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

Published

July 31, 2020, 12:30 p.m.

...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

Published

July 16, 2020, 1:15 p.m.

...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

Published

Dec. 7, 2020, 2:53 p.m.

...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

Published

May 30, 2019, 12:44 p.m.

...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

Published

Sept. 6, 2018, 12:02 p.m.

... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

Published

Sept. 28, 2017, 2:01 p.m.

...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...
MXS Chaining

Type

Blog Post

Published

Feb. 7, 2017, 3:30 p.m.

...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...
Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

Published

July 27, 2023, 1:15 p.m.

...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

Published

Sept. 6, 2022, 1:15 p.m.

.... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

Published

Oct. 22, 2015, 2:30 p.m.

... strain for screening), or arrayed on a 96 well plate where each well contains a defined prey fusion. ...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

Published

Oct. 14, 2015, 2:30 p.m.

...confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Comparing Cpf1...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

Published

Aug. 20, 2020, 2:30 p.m.

... an intrinsically high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059...
Hot Plasmids - August 2020

Type

Blog Post

Published

Aug. 18, 2020, 1:15 p.m.

...systems at Addgene! Schematic of the evolution platform to generate TEV protease variants. Catalysis by...

Showing: 221 - 240 of 271 results

Results per page:

Truncated gRNAs for Regulating Gene Expression

Production of Virus in Insect Versus Mammalian Cells

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Addgene's Cancer Collection Pages Connect the Research Community

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

CRISPR 101: RNA Editing with Cas13

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Isolating Genomic Regions of Interest with the CRISPR System

CRISPR 101: Ribonucleoprotein (RNP) Delivery

AAVs CREATed for Gene Delivery to the CNS and PNS

MXS Chaining

Viral Vectors 101: The Retroviral Lifecycle

Negative Can Be Positive: Open AAV Data with Addgene

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Hot Plasmids - August 2020

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