We narrowed to 308 results for: reporter

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown that the SAM system is robust...

3. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   Published

   Sept. 3, 2020, 12:15 p.m.

   ...system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors encoding...seen in the figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated...

4. 27 Hot Plasmids from 2016

   Type

   Blog Post

   Published

   Dec. 22, 2016, 3:03 p.m.

   ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...

5. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

7. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...donors such as fundraising materials and progress reports, in addition to articles for the Fred Hutch website...

8. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   Published

   Oct. 1, 2019, 1:21 p.m.

   ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...

9. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

10. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...

11. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...problem, you might even be expected to write up a report to communicate your findings to your colleagues...

12. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

13. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...your western blot several times — some researchers report up to ten cycles! Just remember that a bit of sample...

14. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...

15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

16. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...

17. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...

18. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

19. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...Associate Professor at Boston College. National reports stress the importance of providing authentic research...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...sigh. The problem is that many of these studies report correlations without clearly testing whether a ...