...PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely useful but is...such as in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...replicates. ddPCR can be used to quantify DNA sequences that are rare, for example, rare alleles or mutations...

... repeating elements anymore. The kit contains sequences for several different fluorescent proteins. Visualizing... of work on wheat rusts and we are collecting sequences that will provide a similar resource for those...they aren’t sure how to convert their own coding sequence to the MoClo system. It’s quite straightforward...

...pH can denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...

...scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental data tables...query screen data without the need to analyze raw sequence data.  This format is inherently flexible and ...

...series. Single DSBs resolved by NHEJ at coding sequences often result in loss-of-function frameshift mutations...analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting to detect. Another...

...targets 18,080 human genes with 64,751 unique guide sequences to enable both negative and positive selection...proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips and tricks Wang offers this...

...Pennsylvania independently searched for this unique sequence of DNA and both identified lymphocyte antigen ...as a receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was ...alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to...

...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

...antibodies will come with access to the antibody sequences, in line with the rest of Addgene’s recombinant...

...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...

... predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only if present....

...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...

...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

...pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case study...

.... Different antibodies bind different protein sequences called epitopes and scientists can use these to...different targets, the scientists fused them to sequences called complementarity determining regions (CDRs...

...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...