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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 693 results for: ust

Showing: 221 - 240 of 693 results

  1. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like...modular optogenetics tool The researchers also showed just how flexible their LITE system could be by applying...and epigenetic states." Nature. 500, 472–476 (22 August 2013).   , with the addition of histone modifiers...

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... produce monoallelic deletion clones. Cells with just a single intact copy at a given locus may be useful...in unprecedented fashion. Targeted deletions are just one of many available strategies by which biologists...Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR...

  3. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...2020, Addgene's Europe office no longer handles customer support inquiries. The office is now focused on...discoveries. At your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach...worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration. This...

  4. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...Department at Iowa, but kept up a freelance writing hustle on the side. “I actually stumbled upon, about 6...’s persistence paid off: “I spent about 6 months just editing and searching for a job and I stumbled upon...experiment with new careers while in grad school And just for good measure, here’s a few other posts about...

  5. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... etc.). To individually isolate a protein, there must be a special feature about that protein that no ...function for downstream applications. Tag cleavage must be performed after primary purification and requires... tags can also be used for protein purification, just as affinity tags can sometimes be repurposed for...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...voltage indicator (GEVI) called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating...Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... SB transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and...generation of stable transgenic cell lines which can robustly express your gene-of-interest, either constitutively...hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet....

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...how much has been done with CRISPR technology in just 2 years and excitement about how much more there...synthetic biology. I've been answering biology customer service questions for nearly 7 years, and my colleagues...represented engineering first and foremost, from the custom software used to design complex genetic circuitry...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...retroviral backbone. This is considered to be a more robust expression system and has its advantages; however...cassette and visualization vehicle to conduct your custom experiment. Fly vectors The vast majority of fly...guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... the technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

  11. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...sequences from a shared database of custom common features. Second, customers have asked for a way to annotate... involves iterative refinements in response to customer feedback. An example of this approach is SnapGene...

  12. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...can predict whether a gene is natural or synthetic just by looking at its sequence. MIT collaboration for...codons also are not good predictors because they are just that: rare. “Nature has many genes where there are...engineered DNA. Both of these groups have generated robust tools that have practical applications in monitoring...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...begins, enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids...will produce many plasmid copies and others produce just a few copies depending on how they are regulated...give higher copy numbers since the bacteria have just reached stationary phase, but the cells have not...

  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...The WPRE is located at the end of the AAV genome, just upstream of the final ITR.  polyA  The polyadenylation...The polyA is located at the end of the AAV genome, just upstream of the final ITR and downstream of the ...

  16. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...   Fig. 2: The structure of a story - not just for fiction! Image credit: Kenneth Rowe, under the...relate to. It doesn’t need to be anything fancy; it just needs to capture your audience’s attention enough...

  17. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... or not your idea is viable.  Go to experts you trust and pitch what you hope to build.  Then ask for ... pitch we made, we considered the feedback and adjusted our business model accordingly until we had a ...You need a dedicated team that you know well and trust.  This will allow you to discuss each project and...

  18. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...blots and cloning, I decided to pack it in. I had just started month 8 of my 3 year Post-Doctoral contract...reading for my current sales position interview was just as important. The problem was I knew I had a knowledge... and allows you to work with and alongside the customer. From there a move with either promotions or further...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...creations that may not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial...

  20. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month’s time, we’ve published ...YOU to join the fight against coronavirus We are just at the beginning of understanding how SARS-CoV-2...science, and making it accessible to all people, not just scientists! *If you are requesting plasmids for ...
Showing: 221 - 240 of 693 results